The Expression And Significance Of ACAP1 In Ovarian Serous Cystadenocarcinoma

Objective: The expression of ACAP1(ARF GAP with coiled-coil,ankyrin repeat and PH domains 1)in ovarian serous cystadenocarcinoma was detected and its correlation with clinicopathological factors was analyzed.To determine the relationship between ACAP1 and the occurrence and development of ovarian serous cystadenocarcinoma.Methods: The Oncomine database was used to analyze the m RNA expression level of ACAP1 in ovarian cancer,the c Bioportal database was used to analyze genetic variations in ACAPs genes,and the Genemania database was used to analyze the gene-gene interaction network of ACAPs.Selecting 88 cases of tissues diagnosed as ovarian serous cystadenocarcinoma from the Department of Gynecology,Shengjing Hospital of China Medical University from September 2012 to September 2015,of which 67 cases were matched with ovarian cancer metastases,and 16 cases of normal ovarian tissues were selected as control.The expression of ACAP1 in tissues was studied by immunohistochemical method.The T test was used to analyze the difference of ACAP1 expression between groups.The chi-square test was used to analyze the relationship between the expression level of ACAP1 and clinicopathological factors.Kaplan-Meier was used to analyze the relationship between the expression level of ACAP1 in primary ovarian cancer and overall survival.Cox regression was used to analyze univariate and multivariate clinicopathological factors affecting the prognosis of ovarian cancer.The test level was considered statistically significant if P <0.05.Results: 1.Using the Oncomine database we found that the m RNA expression level of ACAP1 in ovarian cancer tissues was higher than that in normal ovarian tissues.2.Using the c Bioportal database,we found that there were many kinds of gene mutations in ACAP family,and the most genetic variations in ACAPs were amplifications.3.Using the Genemania database,we constructed the gene-gene interaction network of ACAPs,and the five genes most closely related to ACAP family were GULP1 、 ARF6 、RAB11FIP3 、 SLC2A4 and VAMP3;4.The expression level of ACAP1 in ovarian serous cystadenocarcinoma tissue is higher than its expression level in normal ovarian tissue(p=1.34e-7);5.The expression of ACAP1 has no significant relationship with ovarian cancer stage,age,peritoneal metastasis,large net metastasis,and ascites volume(p>0.05);6.The high expression of ACAP1 in the primary tumor is associated with poor prognosis(p=0.037);7.The expression of ACAP1 in ovarian cancer metastases is higher than that in the Expression in primary ovarian cancer(p=4.55e-10).Conclusion: ACAP1 is highly expressed in ovarian serous cystadenocarcinoma,and the expression of ACAP1 in the metastatic is higher than that in the primary.The high expression of ACAP1 in the primary is associated with poor prognosis,suggesting that ACAP1 may be related to the occurrence and development of ovarian serous cystadenocarcinoma,suggesting that it may become a target of new diagnosis and treatment for ovarian cancer.