Study On The Changes Of Neurotransmitter GABA、DA And 5-HT Expression In Plasma Of Children With Epilepsy Comorbidities

Objective: Objective To investigate the changes of plasma γ-aminobutyric acid(GABA),dopamine(DA)and 5-hydroxytryptamine(5-HT)in children with epileptic comorbidities with sleep disorder and their related factors.Methods: A total of 60 children with epilepsy admitted to Shengjing Hospital of China Medical University from December 2019 to October 2020 were selected.The Pittsburgh Sleep Quality Index(PSQI)was used to evaluate the sleep status of all subjects.They were divided into a sleep disorder group(n = 27),a group without sleep disorder(n = 33),and a control group(n = 24)without major diseases such as epilepsy.Fastening peripheral venous blood samples(1.0 ml)were collected from the children in the epilepsy group with the shortest time to the last seizure in the morning,and from the healthy children in the control group(1.0 ml).The levels of plasma GABA,DA and5-HT were detected by ELISA.The relationship between the changes of plasma GABA,DA and 5-HT and sleep disorders was analyzed by statistical methods.Results: 1.Plasma GABA,DA and 5-HT levels in epileptic comorbidities with sleep disorder group were lower than those in epileptic comorbidities with sleep disorder group and control group,with statistical significance(P<0.05).2.There were no significant differences in the levels of neurotransmitters GABA,DA and 5-HT among different types of sleep disorders(P>0.05).3.There were no significant differences in plasma GABA,DA and 5-HT among different age groups(P>0.05).4.There was no difference in plasma GABA,DA and 5HT levels between the sampling time and the seizure time(P>0.05).5.In the children with multifocal epileptic seizures,the levels of GABA,DA and 5-HT in the comorbidities group were lower than those in the epileptic group without sleep disorders,and the differences were statistically significant(P<0.05).In the frontal region,central region and extensive seizure,the differences of plasma 5-HT were statistically significant(P<0.05).It is consistent with the distribution of neurotransmitters.6.According to the head MRI results,there were differences in the plasma GABA,DA and 5-HT levels between the epileptic comorbidities sleep disorder group and the epileptic without sleep disorder group(P<0.05),and there were no differences in the neurotransmitter levels between the two groups in other intracranial lesions(P>0.05).7.Among the children recently infected with different pathogens,there were no significant differences in GABA and DA levels between the epileptic comorbidities with sleep disorder group and the epileptic group without sleep disorder group(P>0.05),but the differences in 5-HT levels between the two groups were statistically significant(P<0.05)in the children with mycoplasma infection and mixed infection(P<0.05).Conclusion: The plasma levels of GABA,DA,and 5-HT in children with comorbidities of sleep disorders were all decreased,which were correlated with the seizure site and the disease associated with recent infection to some extent,but not with age,seizure time and intracranial lesions.