| Taraxacum kok-saghyz(TKS)is a plant native to the Tianshan valley on the border between China and Kazakhstan.TKS rubber is a good substitute for natural rubber.TKS’s breeding work necessitates the need to screen high-yielding varieties,hence rapid determination of rubber content is essential for the screening.Conventional analytical methods cannot meet actual needs in terms of real-time testing and economic cost.Near-infrared spectroscopy(NIRS)analysis technology,which has developed rapidly in the field of industrial process analysis in recent years,is a green detection technology with obvious merits of fast measurement speed,low cost and no sample loss.This research aims to develop a portable non-destructive near-infrared spectroscopic detection scheme to evaluate the content of natural rubber in TKS fresh roots.Thermal pyrolysis gas chromatography(Py-GC),was chosen as the reference method for the development of NIR prediction model.The standard error of the reference Py-GC used to calibrate the NIRS method was quite low at 2.64%.298 TKS fresh root samples were collected from the Inner Mongolia Autonomous Region of China and the Institute of Genetics of the Chinese Academy of Sciences,and were divided into four batches for experiments based on different sampling methods.Near-infrared spectra were acquired for each batch of samples.Randomly two-thirds of them were selected as the calibration set,the remaining one-third as the verification set,and the partial least squares method was successfully used to establish a good NIR prediction model.The results showed that the best NIR model has R2=0.95 and RPD=5.54,which could better quantify natural rubber biomass in TKS.In addition,a portable near-infrared spectrometer for measuring the rubber content of TKS has been developed,which can be carried to the field for real-time measurement.This study showed that near-infrared spectroscopy could be used with ease for large-scale screening of TKS plants in farmland,and could greatly facilitate TKS germplasm preservation,high-yield cultivation,environment-friendly,high-efficiency and low-cost rubber extraction,and comprehensive advancement of the dandelion rubber industry thereof. |
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