Clinical Effect Of Autohemotherapy On Herps Zoster And Its Effect On Immune Function

ObjectiveBy analyzing the effects of ozone autohemotherapy on peripheral blood T lymphocyte immune function in herpes zoster patients and its clinical efficacy,our research group want to provide a new effective treatment method for herpes zoster patients.Methods1.Our research group collected clinical dates from 60 herpes zoster patients in the pain department of Tai’an City Central Hospital from March 2018 to December 2018.They were randomly divided into three groups.The control group received conventional treatment,including medication and invasive treatment.The experimental group one were treated with ozone autohemotherapy for 3 days in addition to conventional therapy,and the experimental group two were treated with ozone autohemotherapy for 5 days in addition to conventional therapy,and each group included 20 patients.Medications include intra-venous antivirals,oral analgesics,and invasive treatment including paravertebral radiofre-quency pulse therapy and paravertebral nerve block therapy.2.We use visual analogue scale(VAS)to assess the pain intensity,and the values of three time points before treatment,4 days after treatment and 6 days after treatment were recorded.3.Quality of life assessment:We use the brief pain inventory(BPI)to assess the impact on patients’quality of life.On a scale of 0 to 10,0 indicates no impact and 10 indi-cates the most serious impact.4.Our research group use flow cytometry to observe the changes of peripheral blood T lymphocyte subsets CD3~+,CD4~+,CD8~+cells percentage and CD4~+/CD8~+ratio before treatment,4 days after treatment and 6 days after treatment.Results1.There was no significant difference in the VAS score before treatment among the three groups(P>0.05),and the VAS score after treatment was significantly lower than that before treatment,and it was significantly lower at 6d after treatment than at 4d after treatment.Compared with the control group,VAS scores of patients in the experimental groups decreased significantly on each time point after treatment,and VAS scores of patients in the group two decreased more significantly at 6d after treatment than those in the group one,with statistically significant differences(P<0.05).2.Among the three groups,there was no statistically significant difference in BPI scores before treatment(P>0.05).BPI scores after treatment were all lower than those before treatment,and the BPI scores at 6d after treatment were significantly lower than those at 4d after treatment.Compared with the control group,BPI scores of patients in the experimental groups decreased significantly on each time point after treatment,and the differences were statistically significant(P<0.05),and there was no significant difference between the goup one and group two(P>0.05).3.Before treatment,there was no statistically significant differences in CD3~+,CD4~+,CD8~+cells percentage and CD4~+/CD8~+ratio among the three groups(P<0.05).After treat-ment,the indexes of CD3~+,CD4~+cells percentage,CD4~+/CD8~+ratio all increased and CD8~+cells percentage decreased compared with those before treatment.The changes of CD3~+,CD4~+and CD4~+/CD8~+were more obvious at 6d after treatment than at 4d after treatment.The increase of CD3~+,CD4~+and CD4~+/CD8~+was more obvious in the experi-mental group than in the control group,and the decrease of CD8~+were more obvious.The changes in group two were more significant than those in group one,and the differences were statistically significant(P<0.05).ConclusionOzone autohemotherapy can increase the percentage of CD3~+and CD4~+T lymhocytes and decrease the percentage of CD8~+cells,which can significantly improve the immune function of patients with herpes zoster,reduce their pain and improve their quality of life.