Correlation Between Abnormal Expression Of KRAS,PIK3CA,APC,TP53 Genes And Clinical Characteristics Of Colorectal Cancer Patients

Objective:To study the correlation between KRAS,PIK3 CA,TP53,APC gene mutations and clinical characteristics of patients with colorectal cancer,and to analyze whether there is a correlation between abnormal mutations of different genes in clinical characteristics.Methods:The KRAS,PIK3 CA,TP53,APC gene mutations and clinical characteristics of 60 CRC patients who underwent strict inclusion and exclusion criteria were counted,including the history of current disease（sex,age）,past history（family history,history of hypertension,history of diabetes,history of heart disease,Smoking history,drinking history）,preoperative auxiliary examination results（hemoglobin,BMI,serum albumin,CEA,CA199,CA242）,surgically resected tumor pathology（tumor location,gross type,histological type,degree of differentiation,T stage,N stage,M stage,pathological stage,presence or absence of vascular invasion and neural invasion）.SPSS26.0 statistical software was used for data analysis to analyze the correlation between KRAS,PIK3 CA,TP53,APC gene mutations and clinical characteristics of patients with colorectal cancer,and whether the four gene expressions were correlated with each other in clinical characteristics of patients.Results:1.Among the 60 CRC cases,the genes with high to low mutation rates were APC,P53,KRAS,and PIK3CA（APC mutation rate was 75%,TP53 mutation rate was 53.3%,KRAS mutation rate was 50%,and PIK3 CA mutation rate was 11.7%）.2.There are significant differences in KRAS mutation between different tumor tissue types: compared with KRAS wild-type patients,KRAS mutant patients have more mucinous adenocarcinoma in tumor tissue types（P<0.05）.3.There are significant differences in APC mutation among different tumor tissue types: compared with patients with normal expression of APC gene,there are fewer mucinous adenocarcinomas in the tumor tissue type of APC gene mutation patients（P<0.05）.4.There are significant differences between TP53 mutation and CA199 positivity and different tumor T stages: compared with patients with normal TP53 expression,the CA199 positive rate of TP53 gene mutation patients is higher（P<0.05）;TP53 gene mutation patients have tumors In T staging,more patients were in stage Ⅲ（P<0.05）.5.Regarding the correlation study between KRAS gene and APC gene in clinical characteristics,we have obtained the following conclusions: in KRAS gene mutation patients,APC gene mutation is more common in patients without hypertension（P < 0.05）;KRAS wild type patients Among the patients with APC gene mutation type,the CEA value ≥5 was more（P<0.05）,the tumor histological type was more adenocarcinoma（P<0.05）,and the tumor differentiation type was more moderately differentiated（P<0.05）;APC gene mutation Among the patients,the KRAS gene mutation patients had more primary tumors located in the colon and less located in the rectum（P<0.05）,and the tumor histological type was more likely to have mucinous adenocarcinoma（P<0.05）;In patients with normal expression of the APC gene,the patients with KRAS gene mutation had more CEA value ≥ 5（P < 0.05）,the tumor differentiation of patients was more moderately differentiated（P<0.05）.6.Regarding the correlation study between KRAS gene and TP53 gene in clinical characteristics,we have obtained the following conclusions:among KRAS gene mutation patients,TP53 gene mutation patients have more CA199≥37（P<0.05）;KRAS gene wild-type patients TP53 mutation was more frequent in patients without smoking history（P<0.05）,and the median tumor N stage of TP53-mutant patients was more at stage Ⅱ（P<0.05）;However,in the normal expression type and mutant type of TP53,KRAS gene mutation was not significantly associated with clinical characteristics.7.In the study of the correlation between APC gene and TP53 gene in clinical characteristics,we have obtained the following conclusions:Among patients with APC gene mutation,TP53 gene mutation patients have more CA199≥37（P<0.05）,and more primary tumor is located in the left half colon than in the rectum（P<0.05）,and more differentiated in the degree of tumor differentiation（P < 0.05）;In patients with normal APC gene expression,the patients with TP53 gene mutation had more tumor N stage in Ⅱ stage（P < 0.05）,more prone to nerve invasion（P < 0.05）;In patients with TP53 gene mutation,the degree of tumor differentiation in patients with APC mutation was more moderately differentiated（P < 0.05）.In conclusion:1.There are more mucinous adenocarcinomas in the tumor tissue typing of KRAS mutant patients,but less mucinous adenocarcinomas in the tumor tissue typing of APC mutant patients.2.The positive rate of CA199 in patients with TP53 gene mutation is high,and the tumor T stage of TP53 mutation patients is more in stage III.3.The clinical features of KRAS,APC and TP53 genes have very complex relationship with each other.