| Objective:In this study,liquid chromatography-mass spectrometry（LC-MS）was used to detect serum samples of healthy people,patients with gastric cancer before operation,three months after operation and six months after operation,and patients with recurrent gastric cancer,so as to find out the difference of metabolites between them,and find out the potential biomarkers that can be used to evaluate the curative effect and monitor the recurrence of gastric cancer after further analysis.Method:Using liquid chromatography-mass spectrometry（LC-MS）,metabonomics analysis was carried out on serum of 15 healthy people,serum of 16 patients with gastric cancer before operation,three months after operation and six months after operation,and serum of 15 patients with recurrent gastric cancer after operation,and the corresponding metabolic maps and data were obtained.By comparing the total ion current diagram and principal component analysis results of the samples from the healthy group and the preoperative group of gastric cancer,we can judge whether there are different metabolites between the healthy people and the patients with gastric cancer,and preliminarily screen out the different metabolites between the two groups by T test.Secondly,the differential metabolites screened out above were used to further screen the differential metabolites among the groups of recurrent gastric cancer,preoperative gastric cancer,postoperative three-month group and postoperative six-month group by variance analysis and partial least squares discriminant analysis,and the clustering of differential metabolites among the groups was observed by parallel cluster analysis.It was found that preoperative gastric cancer group and recurrent gastric cancer group were grouped into one group（i.e.tumor-bearing group）,while postoperative three-month group and postoperative six-month group were grouped into one group（i.e.tumor-free group）.According to these two groups,partial least squares discriminant analysis and ROC curve were used to observe the difference between the tumor-bearing group and the tumor-free group,and the difference substance names were found out by using the ratio of cytoplasm to nucleus and HMDB,and finally the cluster analysis was carried out.Result:（1）Comparing the total ion current diagram and principal component analysis of the samples in positive and negative ion mode between the healthy group and the preoperative group of gastric cancer respectively,it can be concluded that there are differences in kurtosis values and different metabolites between the two groups.（2）Seven different metabolites were screened from healthy group,preoperative group,postoperative three months group,postoperative six months group and recurrent group:lysophosphatidic acid,triglyceride,lysine,sphingosine 1-phosphate,phosphatidylcholine,oxidized ceramide and phosphatidylglycerol.（3）It can be seen from the mean chart that lysophosphatidic acid,triglyceride,lysine and sphingosine 1-phosphate are on the rise,while phosphatidylcholine,oxidized ceramide and phosphatidylglycerol are on the decline in the recurrent gastric cancer group,the preoperative gastric cancer group,the postoperative three months group,the postoperative six months group and the healthy group.Conclusion:（1）There are different metabolites in serum between healthy people and patients with gastric cancer;（2）Lysophosphatidic acid,triglyceride,lysine,sphingosine 1-phosphate,phosphatidylcholine,oxidized ceramide and phosphatidylglycerol are different metabolites in the serum of healthy people,patients with gastric cancer before operation,three months after operation,six months after operation and patients with recurrent gastric cancer,and their concentrations are different among different groups of people;（3）Lysophosphatidic acid,triglyceride,lysine,sphingosine 1-phosphate,phosphatidylcholine,oxidized ceramide and phosphatidylglycerol may be potential biomarkers for postoperative curative effect evaluation and recurrence monitoring of gastric cancer. |
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