Experimental Study On The Treatment Of Lycium Barbarum Polysaccharide-glycoprotein In Periodontitis

Periodontitis is a chronic inflammatory disease that impairs the integrity of tooth support tissue.Severe periodontitis not only leads to tooth loss,but also increases the risk of systemic diseases such as atherosclerosis,rheumatoid arthritis,aspiration pneumonia and cancer.The repair and treatment of periodontal tissue such as alveolar bone and periodontal membrane has been a hotspot in the oral field.Periodontal ligament stem cells（PDLSCs）,as seed cells,are widely used in regeneration and restoration in the field of oral engineering.Glycopeptide is a glycoconjugate composed of Chinese wolfberry polysaccharide and five different proteins.Studies have shown that it has antioxidant effects and improves immunity.At present,reports on the treatment of Lycium barbarum glycopeptide（LbGP）in oral diseases at home and abroad are few.Therefore,this study explores the influence of LbGP in vitro and animal experients based on PDLSCs,and hopes to provide new ideas for the treatment and prevention of periodontitis.Objective: Cultivate PDLSCs with LbGP in order to study the effect and mechanism of LbGP on PDLSCs proliferation,migration and osteogenic differentiation,explore the possibility of treating clinical periodontal disease through animal experiments,and provide early theoretical support for the application of LbGP in the oral cavity.Methods: 1.PDLSCs were extracted from the third molars（patients informed and consent）,and characterized by Flow Cytometry,alizin red and oil-red O staining.Then culture PDLSCs with different concentrations of LbGP to explore the roles of LbGP on cell proliferation,migration,inflammatory factors,osteogenic differentiation and potential regulatory pathways.2.The temperature-sensitive hydrogel equipped with LbGP was prepared by chemical synthesis,and the gelling temperature,coagulation time and slow release were determined.3.A rat periodontitis model was constructed using LbGP temperature-sensitive hydrogel to evaluate periodontal tissue regeneration by Micro-CT,HE staining,osteoclast staining,and immunohistochemical staining.Results: 1.PDLSCs have typical mesenchymal stem cells,expressed CD90 and CD29,and have the potential of osteogenic and adipogenic differentiation;LbGP can inhibit the expression of pro-inflammatory factors,promote PDLSCs proliferation,migration and osteogenic differentiation,and promote the bone mechanism related to ERK1 / 2 pathway.2.The temperature and time of the successfully prepared LbGP temperature-sensitive hydrogel meet the use conditions and can be sustained for up to 7days.3.No in vivo toxicity was observed after treatment with LbGP temperature-sensitive hydrogel compared with the control group.And the experiental group significantly reduced alveolar bone resorption,osteoclastogenesis,inflammatory protein IL6 and IL1 expression,and promoted ERK1 / 2 protein expression.Conclusion: LbGP has no in vivo toxicity,which can promote PDLSCs proliferation,migration,osteogenic differentiation,inhibit pro-inflammatory factors and alveolar bone resorption,and its mechanism is closely related to ERK1/2 signaling pathway.This study indicates that LbGP can activate the ERK1/2 pathway to achieve bone facilitation,providing new therapeutic strategies for the prevention and treatment of periodontitis.