Therapeutic Effect And Mechanism Of Lycium Barbarum Polysaccharide-glycoprotein In Amyotrophic Lateral Sclerosis

Objective:Our aim is to observe the therapeutic effect of Lycium barbarum polysaccharide-glycoprotein（Lb Gp）in Amyotrophic lateral sclerosis and to initially explore its possible mechanisms.Method:1.SOD1^G93Atransgenic female mice were randomly divided into saline group（TG-NS,n=16）and Lycium barbarum polysaccharide-glycoprotein group（TG-Lb Gp,n=16）.At the same time,the age and sex matching wild-type female mice were randomly divided into saline group（WT-NS,n=16）and Lycium barbarum polysaccharide-glycoprotein group（WT-Lb Gp,n=16）.Starting from 90 days of age,SOD1^G93Amice were given Lb Gp（20 mg/kg）once daily by intragastric administration until death.2.0bserved the effects of Lb Gp on the body weight,disease onset,lifespan,and disease duration in SOD1^G93Amice.3.Immunofluorescence staining of spinal cord to assess the effect of Lb Gp on astrocytes.4.Evaluate the activation and phenotypic changes of microglia in spinal with immunofluorescence and q PCR.5.Use q PCR to detect changes in the relative expression of inflammatory factors in the spinal cord.Results:Our data suggested that repeated Lb Gp administration prolonged the lifespan,extended disease duration of SOD1^G93Amice by about 17.5%and 35.1%,respectively.Mechanistic studies have shown that Lb Gp inhibit neuroinflammation by inhibiting astrocyte and microglia activation,regulating the phenotype of microglia,inhibiting their polarization to M1 phenotype,and promoting their polarization to M2phenotype.Conclusion:Lb Gp may become a potentially effective treatment for ALS.