Effects Of IcarisideⅡ On Ovarian Cancer SKOV3 Cells And Its Possible Mechanism

Objective: To explore the effects of Icariside Ⅱ on SKOV3 cell proliferation,cell cycle and pyroptosis by regulating ROS/NLRP3/ caspase-1 signaling pathway.Methods: Human ovarian cancer SKOV3 cells were cultured in vitro in an incubator containing 37°C and 5% CO2,and the concentration of Icariside Ⅱ was set as 25,50,75,100,150,200 μmol·L-1 for added intervention.The cells in the logarithmic growth phase were taken.We analyzed the proliferation of SKOV3 cells by icariin Ⅱ at different time periods through CCK-8 The most suitable concentration and time period for the effect of icariin Ⅱ were selected.morphological changes of cell was vision under an upside-down microscopy;cell cycle and intracellular reactive oxygen species were measured by flow cytometry（FCM）;Western blotting was used to determine the expression of apoptosis related genes NLRP3,caspase-1 and IL-1β.Results: 1.Compared with the blank control group,following with concentration increasing.,the proliferation activity of SKOV3 cells could be significantly inhibited,and the effect was significant concentration and time-dependent manners,it had a statistical significance（P < 0.05）.2.After treated with Icariside Ⅱ for 24 h,the number of adherent cells in the intervention group decreased,the cells became flat and round,and grew slowly and floated in large quantities.3.Compared with the control group,SKOV3 cells were arrested in G0/G1 phase after Icariside Ⅱ acted on SKOV3 cells for 24 hours.4.Intracellular reactive oxygen species increased after treatment with Icariside Ⅱ.5.Compared with the control group,the expressions of pyroptosis related proteins NLRP3,caspase-1 and IL-1β in drug intervention group were increased（P < 0.05）.Conclusion: 1.After a period of intervention with a specific concentration of Icariin Ⅱ,it can be observed that human ovarian cancer SKOV3 was significantly inhibited and induced them to arrest in the G0/G1 phase;2.Icariside Ⅱ may induce the pyroptosis of SKOV3 cells by promoting the accumulation of intracellular ROS,and activate the expression of NLRP3,Caspase-1,IL-β genes and proteins.