Design, Synthesis And Application Of Nerve Agent Mimics (DCP And DCNP) And Triphosgene Fluorescent Probes

Diethyl chlorophosphate(DCP)and diethyl cyanophosphate(DCNP)are both organic nerve agent mimics.In the first part of this article,a fluorescent probe based on the FRET mechanism is designed and synthesized to detect DCNP.In this probe,dansylamide was selected as the fluorescence donor,and hemicyanine was the acceptor.The probe showed the acceptor fluorescence at 580 nm in DMF,and the fluorescence intensity at 580 nm in the presence of DCNP Gradually decrease,the fluorescence of dansylamide at 540nm gradually increases,showing obvious FRET phenomenon.Through the time curve test,it is found that the probe can complete the detection of DCNP within 5 minutes,and the fluorescence intensity of the probe does not change significantly under different pH conditions,and it has good stability.Concentration titration experiments found that the probe’s fluorescence change has a certain linear relationship with the concentration of DCNP,and it has good selectivity to common chemical weapon simulants,making it a good DCNP fluorescent probe.The second part is based on the FRET principle to design and synthesize a fluorescent probe for detecting DCP.Since the acceptor part of hemicyanine has a spiro ring structure,it cannot accept the fluorescence of the donor,so the probe is not present in the presence of DCP.,Showed the fluorescence of the acceptor dansylamide at 525 nm.In the presence of DCP,the spiro ring recovered the hemicyanine structure,showing the fluorescence enhancement at 580 nm of the donor,and the detection of the probe can be completed within 3 minutes by the time curve measurement.Concentration titration experiments found that with the addition of DCP,the fluorescence intensity at 525 nm gradually decreased,and the fluorescence at 580 nm gradually increased.In addition,the fluorescence intensity of the probe has a certain linear relationship with different concentrations of DCP.In addition,the probe has a very good selectivity.In the third part,a small organic molecule fluorescent probe was synthesized by modifying the fluoroboron pyrrole matrix,and the amino group was used as the recognition site to detect triphosgene.In the test,it was found that the UV absorption peak at 580nm gradually decreased,a new absorption peak appeared at 520nm,and the fluorescence intensity at 570nm gradually increased,showing a good linear relationship,and good stability at pH 5-10.It also has good selectivity for different ion interferences.