Serum Medicinal Chemistry And Serum Pharmacology Of Nasturtina Extract

The flowers of Trollius chinensis are a kind of detoxificant Chinese traditional medicine with significant anti-inflammatory activity and indicated for respiratory tract infections,tonsillitis,and pharyngitis.It has been demonstrated to mainly contain flavonoids,phenolic acids and alkaloids,but its effective components for anti-inflammatory activity are still unclear,and it will affect the further development and utilization of the flowers of Trollius chinensis,then it is widely believed chemicals that only those who can be absorbed into the body and blood has the possible to become or derive effective ingredient.This paper intends to use serum medicinal chemistry to study the flowers of Trollius chinensis extract's metabolism in vivo,and ues serum pharmacology to analyze activity anaylsis of its' blood transitional components.It's difficuit to analyze because the Chinese medicine components into the bloodstream visa the intestinal absorption are microscale.The main components of the flowers of Trollius chinensis are C-glycosides,and it's difficult to absorb.To solve these two issues,we use a two-step serum pharmaceutical chemistry.The first step is bold serum chemistry after intravenous administration of drugs,analyzed the broad contours of metabolism in vivo,then performe bold serum chemistry after administered orally,and anaylze its' blood transitional components,also investigated the pharmacokinetics contour of the prototype components and metabolism product.This paper use serum pharmacology experimental to study the anti-inflammatory activity of the blood transitional components.Complex the information form those two parts,getting the anti-inflammatory contribution of the flowers of Trollius chinensis prototype components and metabolism product.22 prototype components and 58 metabolism product form bold serum chemistry after intravenous administration of drugs was identified through LC-MSn,and analyzed its'pharmacokinetic circumstances.2 prototype components and 42 metabolism product form bold serum chemistry after oral administration of drugs was identified through LC-MSn too,and analyzed its'pharmacokinetic circumstances.Use the vitro anti-viral model constructedby MDCK cells tested the 12 time points containing serum antiviral activity at different concentrations.The metabolic rules of flavonoids,phenolic acids and alkaloids were concluded.The main metabolic reactions in vivo of pheolic acids were hydrolysis of glucoside and the aglycone can further take place phase ? oxidation reactions such as co-oxidation,epoxidtion,oxidation of aromatic hydrocarbons,O-dealkylation and oxidation of the double bond,the reduction means a carboxyl group reduced to an aldehyde or alcohol.The phase ? contains methylation glucuronide,sulfation,and amino acids conjugation etc.For flavonoids,the hydrolysis was hard to take place,but the phase ? reactions,such as glucuronidation,sulfation,methylation were taken place easily on the parent nucleus of flavonoids,which can increase the polarity of compounds and do some help for their excretion.Flavonoids can generate C6-C3 or C6-C2 derivatives by intestinal bacteria metabolism.Alkaloids' phase ?metabolic include methyl,sulfuric acid,glucuronic acid and acetyl binding reaction,It will happen heterocyclic cracking reaction when occur specific binding reactions in vivo.Each concentration at each time point containing serum and positive drug both acted no cytotoxicity and exhibited antiviral activity checked by antiviral model in vitro.10 min containing serum showed the greatest antiviral activity at all time point has been tested,it related to phenolic acids' phase ? metabolism and Alkaloids' metabolism.