Characterization Of Characteristic Compound Groups Of Chinese Gesneriaceae Medicinal Plants Based On LC-MS

Objective:Gesneriaceae,one of the important groups of Scrophulariales,can be divided into Cyrtandroideae and Gesnerioideae,with 150 genera and 3700 species altogether.There are 58 genera and 470 species,with 28 genera specialty in china,which belong to Cyrtandroideae.Plants of this family are widely used to treat dysentery,bruises,snake bites,arthritis in minority areas of Guizhou,Guangxi,Yunnan provinces.However,most of the plants grow on rocks or ground,and their life is affected by the environment,which caused parts of plants approaching extinction.The chemical constituents of the majority plants are still in the blank.The revealed chemical constituents included flavonoids,benzene glycosides,terpenoids,quinones,etc.and they possess strong pharmacological activity.In order to grasp our resources of Gesneriaceae medicinal plants,define its chemical constituents,screene exploitable species and make good use of the resource.The studies are conducted as follows(1)Analysing phenylethanoid glycosides with liquid chromatography-mass spectrometry analysis to summarized the characteristics of various types of cleavage of phenylethanoid glycosides.(2)Making extraction and LC-MS determination of the collected sanmple,then establish the chemical constituents analytical method,combing the cleavage of phenylethanoid glycosides to characterize chemical constituents of Gesneriaceae,phenylethanoid glycosides(3)Using PCA and OPLS-DA to analyze of the difference component of Lysionotus D.Don and Hemiboea Clarke to explore the chemical relationship between them.Method1.By consulting large number of documents,this paper make a comprehensive review of the chemical composition and pharmacological activity of Gesneriaceae plants.2.An appropriate method is established for the analysis of 8 kinds of phenylethanoid glycosides using ultra performance liquid quadrupole time of flight mass spectrometry instruments(UPLC-Q-TOF-MS),so that all the ingredients have a good separation,optimizing MS conditions so that information of cleavage fragments of each component is stable.3.According to primary,secondary mass information,combined with the compound database,DAD characteristic spectrum,compound ClogP value and other information,we make an overall characterization and identification of 24 Gesneriaceae samples.4.PCA and OPLS-DA were used to analyze of the difference component of Lysionotus D.Don and Hemiboea Clarke.Results and conclusion1.Analysing the primary,secondary mass information of the 8 phenylethanoid glycosides standard and summarizing fragmentation way different types of phenylethanoid glycosides;making a valid distinction of C-4’ position and C-6’ position Caffeoyl in phenylethanoid glycosides,phenylethanoid single glycoside containing glucose or rhamnose;phenylethanoid glycoside containing rhamnose-glucose or rhamnose-apiose,C-3’position and C-6’ position glycosylation in phenylethanoid glycosides.2.Gesneriaceae plant samples were analysed through UPLC-Q-TOF-MS.According to the reference fragmentation way,database,and Chemspider,Scifinder to get possible formulas candidate compound.Then through secondary mass information,DAD characteristic spectrum and compound ClogP value to make identification of the compounds.Results show that Gesneriaceae plants contain phenylethanoid glycosides,flavonoids and other types of compound,differences of typesand contents of phenylethanoid glycosides exists between different species of gesneriaceae.3.Using PC A and OPLS-DA to analyze the difference component of Lysionotus D.Don and Hemiboea Clarke.and 13 markers were selected between them,most of which are phenylethanoid glycoside.The results indicate that chemical components of Lysionotus D.Don and Hemiboea Clarke have similarities,but the distinctions between them are obvious as well.