Effects Of Weak Laser Light On The Homeostasis Of Reactive Oxygen Species In CCC-ESFs Under High Glucose

Diabetes mellitus is a chronic degenerative disease characterized by hyperglycemia.Among the complications associated with diabetes,delayed wound healing is the most serious problem in diabetic patients,which can lead to amputation.At high glucose concentration,excessive reactive oxygen species are produced due to the imbalance between intracellular oxidation and antioxidation,and antioxidant expression decreases,protein structure changes,cell proliferation inhibition and apoptosis increase.It causes cells to be damaged by toxic oxidative stress,which leads to inflammatory reaction and poor wound healing.Photobiomodulation can not only increase the activity of antioxidant enzymes,regulate the balance of antioxidant system,accelerate collagen synthesis,but also promote the secretion of cytokines and growth factors,reduce inflammatory reaction and promote cell proliferation.it can promote wound healing in many ways,so it can be used as a method for the treatment of diabetic wound healing.The purpose of this study was to study the effect of photobiomodulation on the homeostasis of mitochondrial reactive oxygen species in human embryonic skin fibroblasts under high glucose environment.Human embryonic skin fibroblasts were cultured in vitro and divided into control group,635 nm laser irradiation group and808 nm laser irradiation group.The energy density of the experimental group was 1.5,3,6,12 J/cm~2.After 2 d of high glucose modeling,the control group was not exposed to light.The experimental group was irradiated with laser for 3 d,and the cell proliferation was detected by MTT method.The results showed that laser irradiation could promote cell proliferation,and the effect was better in 635 nm and 20 m W/cm~2.Then the expressions of reactive oxygen species,total superoxide dismutase and total antioxidant capacity in cells were detected.The results showed that high glucose caused oxidative stress damage to cells by inducing high levels of reactive oxygen species to destroy the balance between oxidation and antioxidation.Photobiomodulation can improve the antioxidant capacity of cells,reduce the level of high concentration of reactive oxygen species induced by high glucose,and restore the balance between oxidation and antioxidation.Finally,the levels of mitochondrial membrane potential,nuclear transcription factor,tumor necrosis factor and interleukin-1?were detected.The results showed that photobiomodulation could attenuate apoptosis induced by high glucose and regulate inflammatory state.