| In a high-altitude,low-pressure and low oxygen environment,low oxygen often occurs in various systems and organs of the body,and the cardiac muscle is one of the tissues that are sensitive to low oxygen in various systems and organs of the body.Cardiac muscle tissue maintains the body’s blood circulation and guarantees the body’s oxygen supply,but the heart is also a tissue and organ that consumes more oxygen and is very sensitive to low oxygen.If you do not intervene in time for treatment,the harm will be particularly great.Therefore,increasing the research on the prevention and treatment of cardiac muscle injury induced by low pressure and low oxygen in rats and enriching its treatment methods is of great significance for reducing the degree of cardiac muscle tissue damage.The raw material for plaster acupuncture therapy is high-quality Sibin vermiculite,and combined with contemporary advanced superfine powder coating technology,the plaster acupuncture powder is mixed with low-sensitivity medical pressure-sensitive adhesive to develop an innovative treatment that is safe and non-toxic.It has many advantages such as pain and timeliness.In this study,plaster acupuncture therapy was used to stimulate the acupoints "Baihui" and"Neiguan" in rats to observe the effect of this therapy on rat heart function,endothelial cell activity and aquaporin expression level,and explore the effect of plaster acupuncture therapy on low pressure and low oxygen cardiac muscle injury The prevention and control effect.ObjectiveIn this study,the changes of endothelin-1(ET-1)and ANP in serum and cardiac muscular tissue of rats and the expression level of AQP-1 in cardiac muscular tissue and the influence on the pathomorphology of cardiac muscular tissue were observed to explore the protective effect of needle sticking therapy on rats with acute cardiac muscular injury.Method1.Experimental animal grouping:45 SPF adult male SD rats were randomly divided into 5 groups:normal group,model group,model+biantie group,model+positive drug group and normal+biantie group,with 9 rats in each group.2.Establishment of acute hypoxia model:the hypobaric oxygen chamber was used to simulate the high altitude environment,and the simulated altitude was 6000 m.The model group,model+biantie group and model+positive drug group were placed in the chamber for 24 hours,and the hypobaric hypoxia model was established by fasting and water deprivation during the period of entering the chamber.3.Intervention methods:the normal group and the normal +biantie group were fed normally in the normoxic environment,and the normal+ biantie group were treated with square biantie(3*3 mm)for 1 h and fixed with adhesive tape every day for 14 days;The rats in the model group were pasted with adhesive tape for 1 hour a day,the rats in the model+Bian paste group were pasted with square Bian paste(3*3 mm)for 1 hour a day and fixed with adhesive tape,and the rats in the model+positive drug group were intragastrically given Dazhu Hongjingtian capsule 280 mg/kg once a day according to the weight of rats.The rats in the three groups were placed in the simulated high altitude hypobaric oxygen chamber for 24 hours on the 15th day.The rats in the five groups were deprived of food and water for 24 hours on the 15th day.4.Appearance observation:after modeling,the rats were observed from the aspects of weight,appearance and reaction,and the changes between different groups were compared.5.Histomorphological observation:after the end of the model,the rat heart was taken out,the apical part of the heart was cut and placed in the fixed solution,the sections were prepared,the hematoxylin eosin staining was performed routinely,and the histomorphological changes were observed under the light microscope.6.Detection of blood biochemical indexes:after modeling,the blood was taken from the apex of rat heart,and the serum was centrifuged.The contents of ET-1 and ANP in the serum were determined by enzyme-linked immunosorbent assay.7.Cardiac muscular tissue index detection:after modeling,the rat heart was taken out,and the rest of the heart was stored in a cryopreservation tube.The contents of ET-1 and ANP,the expression levels of AQP-1 mRNA and AQP-1 protein in rat cardiac muscular tissue were detected by enzyme-linked immunosorbent assay,RT-PCR and Western blot.Result1.Comparison of general conditions:after modeling,the rats in the model group lost weight,had slow response,white ears and faster respiratory rate;The performance of Bian tie group and drug group was acceptable.2.Results of H.E staining:the cardiac muscular tissue sections of rats showed that the cardiac muscular tissue of normal group and normal+biantie group mainly showed full cytoplasm,clear nuclear margin,tight and orderly arrangement of muscle fibers,and no obvious hypoxic lesions.In the model group,the main manifestations of myocardium were nuclear atrophy and necrosis,cytoplasmic shrinkage,loose and disordered arrangement of muscle fibers,wavy arrangement,and obvious pathological changes of hypoxia injury;Under the intervention of biantie and drugs,the hypoxic pathological changes of cardiac muscular tissue in model+biantie group and model+positive drug group were significantly improved,and the overall arrangement of muscle fibers was good,close and orderly.3.The results of blood biochemical index test:the results of this experiment showed that the ET-1 and ANP contents in the model group were significantly higher than that of the normal group(P<0.5),and there was no significant difference between the ET-1 and ANP in the normal+Bian stick group;Compared with the model group,ET-1 and ANP content in model+Bian stick group and model+positive drug group were significantly decreased(P<0.05);There was no significant difference between the serum ET-1 and ANP in the model+Bian patch group and the model+positive drug group.4.Biochemical indexes of cardiac muscular tissue:4.1.The results of ET-1 and ANP in cardiac muscular tissue showed that compared with the normal group,the contents of ET-1 and ANP in the model group were significantly increased(P<0.05),but there was no significant difference in the contents of ET-1 and ANP in the normal+biantie group;Compared with the model group,the contents of ET-1 and ANP in model+biantie group and model+positive drug group were significantly lower(P<0.05);There was no significant difference in the contents of ET-1 and ANP between the model+biantie group and the model+positive drug group.4.2.the results of AQP-1mRNA expression in cardiac muscular tissue showed that the expression level of AQP-1mRNA in model group was significantly higher than that in normal group(P<0.05),and there was no significant difference in AQP-1mRNA expression in normal+Bian group;Compared with the model group,the expression of AQP-1mRNA in model+Bian patch group and model+positive drug group was significantly lower(P<0.05);There was no significant difference in AQP-1mRNA expression between model+Bian patch group and model+positive drug group.4.3.The protein expression of AQP-1 in cardiac muscular tissue showed that compared with the normal group,the protein expression level of AQP-1 in the model group was significantly increased(P<0.05),and there was no significant difference in the protein expression level of AQP-1 in the normal+ biantie group;Compared with the model group,AQP-1 protein expression level in model+biantie group and model+positive drug group was significantly lower(P<0.05);There was no significant difference in the protein expression of AQP-1 between model +biantie group and model+ positive drug group.Conclusion1.Biantie acupoint pretreatment can effectively reduce the content of ET-1 and ANP in serum and myocardial tissue,thus improving the degree of vascular endothelial cell damage and cardiac diastolic function.2.Biantie acupoint pretreatment can reduce the expression levels of AQP-1 mRNA and AQP-1 protein in cardiac muscular tissue of rats,so as to improve the degree of cardiac muscular edema.3.Biantie acupoint pretreatment has obvious protective effect on acute cardiac muscular injury caused by acute hypobaric hypoxia in rats.Its protective effect may be related to the improvement of vascular endothelial cell injury,maintenance of cardiac diastolic function and reduction of cardiac muscular edema. |
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