Construction And Characterization Of Fennel Essential Oil Microemulsion System

As a fennel extract,fennel essential oil is composed of active substances such as anethole,coumarin and terpene lactone.Although it is a medicinal and edible material with fragrant and antioxidant properties,the range of applications of essential oils is limited due to its low solubility in water,low volatility and low rate.absorption and use.In order to solve the above issues and expand the scope of fennel essential oils,this article optimizes the process of extracting fennel essential oils by response surface methodologies,builds essential oil microemulsion systems of diluted fennel and their physical properties and which systematically study some of the chemistry..The main content is as follows.(1)Using the yield of fennel essential oil as an index,the effects of extraction solvent,liquid-to-liquid ratio,extraction time,particle size and extraction temperature on The preparation of fennel essential oil by the sock method have been studied,and the extraction process has been analyzed by response surface experiments.Optimize.As a result,the optimal process parameters for extracting fennel essential oil with a Soxhlet extractor were:The material/liquid ratio is 1:8.4,the extraction time is 4.09 hours,the particle size is 80 mesh and the extraction rate of essential elements from fennel is as follows:The oil under these conditions was 15.99%.(2)The essential oil of fennel is prepared by pseudo three-phase projection.Fennel essential oil is used as the oily phase,and the area ratio of the microemulsion surface area and the minimum dilution ratio(DR)of the pseudo-ternary phase diagram are used as indicators.The formulation and dilutable preparation process is optimized and the phase properties of the microemulsion system are analyzed using conductivity and rheology methods.The results showed that two formulations of fennel essential oil microemulsion diluted with an average particle size of 28.39 ± 0.53 nm were obtained,respectively.Tween 80+40/glycerol/fennel essential oil=4:2:1.5 and tween 80+40/glycerol/fennel essential oil=6:3:1;if the water content of the fennel essential oil microemulsion is lower,the 48%microemulsion is of the W/O type.If the moisture content exceeds 62%,the microemulsion is of the O/W type.water is 48%to 62%,the microemulsion is of type BC.(3)The stability of the fennel essential oil microemulsion was studied.Through experiments with antioxidant activity in vitro,the antioxidant capacity of fennel essential oil microemulsions was studied.Following the principles of chemical kinetics,Schaal’s accelerated oxidation method was used to study the effect of storage temperature on fennel.A model for predicting the effect of essential oil microemulsions on the oxidative stability and shelf life of fennel essential oil microemulsions was established.The results show that the light transmittance of fennel essential oil microemulsion can be maintained above 90%after centrifugation at 3000-7000 rpm for 30 minutes.The fennel essential oil microemulsions have a maximum temperature of around 80℃ and are heated below 80℃.At 30 minutes,there was no significant difference in particle size and PDI value compared to to the control group.Microemulsions stored at 25℃ are the most stable.There is no significant difference in particle size and PDI value compared to the control group when stored for 90 days.The semi-inhibitory concentrations(IC50)of fennel essential oil and fennel essential oil microemulsion for DPPH free radical scavenging capacity were 2.02 mg/mL and 1.74 mg/mL,respectively.The IC50 values for OH free radical scavenging capacity were 4.28 mg/mL and 3.47 mg/mL.The IC50 values for O2 free radical uptake were 4.28 mg/mL and 3.47 mg/mL.The total reducing power IC50 values are 5.23 mg/mL and 4.59 mg/mL,respectively,indicating the antioxidant properties of the microemulsions.The oxidizing capacity is slightly better than a single essential oil.The fennel essential oil microemulsion oxidation rate model belongs to the first order rate equation(lnC=C0-kt),with an R2 greater than 0.97.The Arrhenius equation established on this basis was:k=2518.85·e-35583.09/RT The model predicts that the theoretical shelf life of fennel essential oil microemulsion at 20℃ is about 80 days.