Effects Of Surfactant Exposure On The Skin Of Young College Students And Restorative Effects After Lipid Mixture Intervention

ObjectiveThis study using lipidomic tools to explore changes in skin surface lipids(SSLs)profiles of college students’ hands after exposure to detergents.In addition,after the exposure experiment,all subjects carried out a total of 7 days of intervention experiments based on their self-control,in which the right hand was the experimental group,and a physiological lipid mixture with linoleic acid-ceramide as the main component was applied.The left hand was the control group,applied a glycerin moisturiser that removes physiological lipids.By detecting the changes in the SSLs spectrum at different stages of intervention,it provides a theoretical basis for early intervention protocols after acute exposure to detergent solutions.MethodsThis study was a self-controlled study.The research subjects were recruited by on-site recruitment.According to the inclusion and exclusion criteria,a total of 46 young college students were finally included,all from Zhengzhou,Henan.The left hand of all subjects was the control group,and the right hand was the experimental group.According to the study design,the exposure experiment was firstly performed with detergent solution containing surfactant(concentration 2%,PH=11),and then the intervention experiment was performed with lipid mixtures of different main components.At different stages of the study,samples of SSLs were collected and SSLs were determined by ultra-high performance liquid chromatography time of flight mass spectrometry(UPLC-QTOF-MS).Principal component analysis(PCA)was used to assess the degree of variation within and between groups of SSLs profiles.Orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen for substances that significantly contributed to the differences between groups.Use the metabolite enrichment analysis(MSEA)algorithm in Metabo Analyst 5.0 to determine the lipid metabolism pathways that are mainly enriched for important contributing substances.ResultsA total of 46 subjects were included in this study,including 23 males and 23 females,with an average age of 24.02±1.80 years.1.The average abundance of SSLs decreased after detergent exposure,and cholesterol lipids,glycerophospholipids,fatty acids,and sphingolipids were considered to be characteristic damaged lipids.2.Compared with exposure,the mean abundance of SSLs in both the experimental group and the control group continued to decrease after 1 day of the intervention.At this time,the average abundance of SSLs in the experimental group was lower than the control group,and only the content of precursor substances of some SSLs was higher than the control group,mainly sphingosine,diglyceride,and long-chain fatty acids.3.Compared with exposure,the average abundance of SSLs in the experimental group began to gradually recover after 7 days of intervention,while the average abundance of SSLs in the control group continued to decrease.At this time,the average abundance of SSLs in the experimental group was higher than in the control group.Glycerolipids,fatty acids,and sphingolipids were considered to be the major recovered lipid species.4.Compared with 1 day after intervention,the average abundance of SSLs in the experimental group increased after 7 days of intervention(except glycerophospholipids),while the average abundance of SSLs in the control group continued to decrease.Partial sphingolipids,glycerolipids,fatty acids,and steroid lipids were considered to be the major types of lipids recovered durthe ing long-term repair.Conclusions1.Exposure of hand skin to detergent solutions containing surfactants can damage SSLs.2.After the hands were exposed to detergent,the application of moisturizing cream with glycerin as the main component in the control group could reduce the damage of SSLs in a short term,but the SSLs decreased after long-term application.3.After the hands were exposed to the detergent,the lipid mixture with linoleic acid-ceramide as the main component in the experimental group did not significantly help the recovery of SSLs in the short term,but the SSLs could gradually increase after long-term use.