Effect Of Alkyne-ligand-based Chiral Gold Clusters,Copper Complexes On The Differentiation Behavior Of Mesenchymal Stem Cells(MSCs)

Chirality is one of the most important biochemical features in living organisms,and many biological phenomena and physiological processes depend on the chirality of biomolecules.The interaction of cells with chiral material is an important topic in biological research.Studies have shown that nanomaterials modified by chiral molecules have remarkable results in regulating cells preference for adhesion and cells chirality-dependent differentiation.However,most studies have focused on the chiral effects of chiral small molecules(amino acids and their derivatives)on cell behavior,ignoring the exploration of cell interactions with other chiral molecules(chiral metal complexes or chiral metal clusters).Therefore,it is necessary to investigate the effects of chiral metal complexes and chiral metal clusters,which not only helps to break the limitations of chiral molecular design,but also reveals the importance of chirality in the extracellular matrix(Extracellular matrix,ECM).This thesis uses chiral copper complexes and chiral gold nanoclusters to construct different chiral nanocomposites and explores their effects on the induced differentiation of bone mesenchymal stem cells(Mesenchymal stem cells,MSCs).The specific work content is as follows:In the first chapter,chiral gold nanoclusters protected by a couple chiral glycyl alkynyl(L/D-ligand)is loaded on the surface of a gold nanoparticle film(AuNP film)to form a couple chiral two-dimensional gold film(L/D-film),which were used to regulate the adhesion and differentiation behavior of MSCs.Studies have shown that MSCs on the surface of D-film had a higher cell density,a larger spreading area,and a greater proportion of differentiated osteoblasts.Compared with D-film,the MSCs on the surface of L-film had lower cell density,smaller spread area,and more proportion of differentiated lipoblasts.The results were further confirmed by examining the expression of a marker(RUNX2)in osteoblasts.In chapter two,the R/S-copper complex induced by chiral alkynyl ligand(R/S)-2diyl-2-hydroxyl methylpyrrolidone-l-proyne(R/S-ligand)was introduced into the sodium carboxymethylcellulose(CMC)hydrogel to form the R/S-copper complex carboxymethylcellulose(R/S-Cu-CMC)3D chiral hydrogel material.These chiral hydrogel materials affected on MSCs to regulate their induced differentiation behavior.Studies showed that R-Cu-CMC hydrogel induced more osteogenesis differentiation of MSCs and S-Cu-CMC hydrogels induced more adipogenesis differentiation of MSCs.