Nutrient Composition Research And Product Trial Production Of Polygonati Rhizoma

As a medicinal plant,Polygonati Rhizoma has attracted much attention for its effects in regulating blood sugar,anti-oxidation,and enhancing immunity.At present,the research on Polygonati Rhizoma mainly focuses on its medicinal value;however,as a prescribed type of homology of medicine and food,the main nutritional components of Polygonati Rhizoma and the changes in the components during the processing in the production area are rarely reported.In order to explore the differences in the nutritional components of Polygonati Rhizoma caused by factors such as differences in production areas and technological processing,and to solve the problems of lack of innovation in the development of medicinal and food homologous Polygonati Rhizoma medicinal materials,this paper adopts the uniform experimental design method and the orthogonal experimental design method.Using HPLC technology,through cluster analysis,principal component analysis and multivariate nonlinear regression calculation methods,combined with food sensory evaluation methods,comprehensively evaluate the nutrition and medicinal quality of Polygonati Rhizoma from different production areas,and optimize the screening to obtain the best processing technology of Polygonati Rhizoma and to trial production of Polygonati Rhizoma food,explore the biological activity of Polygonati Rhizoma extract.Effectively improve the development and utilization rate of Polygonati Rhizoma and enrich the variety of medicinal and food homologous Polygonati Rhizoma products.The main results are as follows:1.Comparing the differences in the main nutritional components and medicinal active components of Polygonati Rhizoma from 9 different production areas,the results show that the nutrient content of Polygonati Rhizoma from Zhejiang is significantly higher than that from other areas;according to the correlation analysis results of various chemical components,established the quality evaluation system of Polygonati Rhizoma comprehensive scoring method in different production areas,and obtained the best quality of Polygonati Rhizoma produced in Jingning,Zhejiang Province,the score is 51.38.2.The optimal processing process of Polygonatum was obtained by optimization: nine times of steaming and drying according to the ancient method,the optimization is that the number of times of steaming(X1)is 6 times,the steaming time(X2)is 4.75 h,and the drying time(X3)is 4h,the drying temperature(X4)is 60°C,and the soaking time(X5)is5.5h.The raw material formula of milk-flavored Polygonatum steamed bread was optimized: fixed wheat flour was 100 parts,added purified water 40 parts,Polygonatum powder 20%,pure milk 20%,yeast powder 1%,white sugar 2%.The order of the effect of different raw material additions on the quality of Huangjing steamed bread was: the addition of Huangjing powder>the addition of milk>the addition of white sugar.The raw material formula of Polygonatum buckwheat noodles is optimized: fix wheat flour as 100 parts,add purified water 55%,salt 1.5%,Polygonatum powder 15%,buckwheat flour 5%,active gluten powder 2.5%.The order of size is: the added amount of buckwheat flour>the added amount of water>the added amount of Huang Jing powder>the added amount of active cashew gluten flour.3.The HPLC fingerprints of processed Polygonati Rhizoma were established,and the similarity results showed that the similarity between the processed products of S1-S6 was between 0.862 and 0.984;It is 0.665,indicating that the processing technology has an impact on the chemical composition of Polygonati Rhizoma,and the chemical composition content of processed Polygonati Rhizoma products with different processing times has significant differences;further,the cluster analysis results show that when the distance between groups is 5,S3,S4,S5 is a class,S1,S2 are a class,S6,S7 are a separate class,the increase of processing times has a certain impact on the chemical composition of Polygonati Rhizoma.4.Polygonatum polysaccharide did not have significant cytotoxicity in the concentration range of 10 mg/mL～1 μg/mL;Polygonatum polysaccharide at different concentrations had significant resistance to glucose consumption in 3T3-L1 adipocytes(p<0.05).Both insoluble dietary fiber(IDF)and soluble dietary fiber(SDF)in Polygonati Rhizoma have obvious antioxidant activities in vitro,and the ABTS+˙ free radical scavenging rate of Polygonati Rhizoma SDF is significantly enhanced after processing,reaching 88.71%;the DPPH free radical of IDF after processing The clearance rate was significantly increased by 6.19%;the total antioxidant capacity of Polygonati Rhizoma dietary fiber was significantly enhanced after processing,reaching 109.56 μmol/g～150.99μmol/g.The insoluble dietary fiber in Polygonati Rhizoma has a certain ability to bind choline salt in vitro,and the binding capacity of glycocholate and taurocholate of IDF after processing increased significantly,reaching 25.41% and 28.47%,respectively(p<0.05).