| Physalis alkekengi L.var.franchetii(Mast.)Makino(PAFM)is a plant of the genus Solanaceae,also known as the red girl.Its fruits can be eaten and the calyx can be used as medicine.Mainly distributed in northeast China,Shanxi and other areas,the medicinal use of PAFM has a long history in China,and the natural products contained in its extract have high biological activity.Gastric cancer is one of the common malignant tumors.Recent years in our country the incidence and mortality of Gastric cancer continue to increase,which is seriously damaging peoples physical and mental health.Traditional therapeutic methods include surgery,radiotherapy,chemotherapy and biological immunotherapy,etc.,but chemotherapy drugs have very limited efficacy in the treatment of advanced gastric cancer with relatively large toxic and side effects.Therefore,the search for natural products that effectively inhibit gastric cancer cells can greatly promote the research and development of new anti-tumor drugs,which has high clinical application value.In this study,27 compounds were preliminarily identified by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS),and the structure of the compounds with higher content in the extracts was identified.A molecular network database was established by LC-MS/MS tandem mass spectrometry and the distribution of physalins in the extracts of PAFM was analyzed.The results showed that physalin A accounted for the highest proportion of physalins in PAFM.In order to explore the effects of the main pharmacodynamic components of the extract of PAFM on gastric cancer cells,we constructed a network of "physalins compounds-gastric cancer-key proteins" through network pharmacology,and used KEGG Mapper to screen out 9 key proteins,conducted molecular docking between the two key proteins.The results showed that,all of the 9 key proteins had good binding ability,and the affinity with cyclin(CDK2)was high.These results indicate that physalin A and gastric cancer cells may have potential interaction proteins.In order to verify the effect of physalin A on gastric cancer cells,we used CCK8,cell cloning and flow cytometry to verify the tumor inhibitory activity of physalin A.The results showed that physalin A had no effect on the growth of gastric epithelial cells GES-1.In addition,physalin A can inhibit the proliferation of gastric cancer cells by inducing AGS G0/G1 cell cycle arrest.These results suggest that physalin A may affect the growth of gastric cancer cells by influencing cyclin.In summary,a total of 27 active components of PAFM were identified,and it was found that physalin A has strong inhibitory effect on gastric cancer cells.This study provides theoretical basis for the development of anti-gastric cancer drugs or health products around physalin A,which has high clinical significance and practical application value. |
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