Construction Of Multifunctional Compound Microparticles And Its Application In Wound Healing Of Diabetes

The treatment of chronic diabetic wounds remains challenging due to the rapid bacterial infection,severe inflammation,and insufficient angiogenesis.Immune cells are a core component of the complex inflammatory environment of wounds.Accelerated angiogenesis can provide oxygen and nutrients to damaged tissues.these determine the repair and regeneration of the skin,which is an effective way to promote the healing of diabetic wounds.To address these challenges,a novel multifunctional composite microparticle was developed by antisolvent-induced co-assembling silk fibroin-ε-poly-L-Lysine microspheres(m SF-EPL)and further assembling m SF-EPL with polydeoxyribonucleotides(PDRN)and Exo.The release results showed that composite microparticles had long-term sustained PDRN and Exo release profiles as well as outstanding release efficiency.Furthermore,in vitro studies have shown that composite microparticle exhibits effectively antibacterial action,inducing antiinflammatory M2 macrophage phenotype,and promoting angiogenesis.In the investigations of diabetic wound healing,composite microparticle promotes granulation tissue formation,collagen deposition,and epithelialization of wound tissue,resulting in significantly accelerating skin healing.Therefore,this work presents a promising strategy for the clinical treatment of chronic diabetic wound.