| From ancient times to the present,there has always been a related concept of"medicine and food homology"in the field of traditional Chinese medicine in China.In recent years,with the improvement of living standards,people pay more and more attention to health care and health preservation,and"medicine and food"substances have gradually entered people’s daily life.These medicinal and edible dual-use substances contain a variety of functional ingredients,and people often pay attention to their efficacy and ignore the safety of their use.Existing studies have shown that the endogenous components in some medicinal and edible dual-use substances will cause certain harm to organisms when used in a certain dose or for a long time.Therefore,the research on new methods for the analysis of some endogenous components in medicinal and food dual-use substances is of great practical significance to ensure the safety of the quality of these substances and guide people to use these substances rationally.Based on this,this paper selects some endogenous components of medicinal and edible dual-use substances Ginkgo Semen,Puerariae Lobatae Radix and Curcumae Longae Rhizoma as research objects.By using ultra-high performance liquid chromatography technology,new methods for the analysis of endogenous components in the above-mentioned medicinal and edible dual-use substances were established and applied to the analysis of actual samples from different regions in China,and achieved good results,which provides a scientific basis for the safety risk assessment of the above-mentioned medicinal and edible dual-use substances in the future,and also provides technical support for the effective quality monitoring.The main research results are as follows:1.A new analytical method for the simultaneous determination of pyridoxine and pyridoxal in Ginkgo Semen was established by ultra-high performance liquid chromatography-fluorescence detection technology(UPLC-FLD).The Ginkgo Semen samples were extracted with 0.10 mol/L hydrochloric acid,and the extraction solution was purified by ZIF-8 sorbent,and the purification conditions were optimized.Under the optimized experimental conditions,pyridoxine and pyridoxal had a good linear correlation(R~2≥0.9999)in the concentration range of 0.03～10mg/L,and the detection limits of pyridoxine and pyridoxal were 0.0065 mg/L and0.0057 mg/L,respectively.The recovery rate of pyridoxine and pyridoxal in Ginkgo Semen was 86.2%～110.4%with the relative standard deviation less than 7.5%.The established method was used to measure Ginkgo Semen samples from nine different regions in China,and good results were obtained.The contents of pyridoxal and pyridoxine in Ginkgo Semen ranged between 0.39～1.26 mg/kg and 0.56～26.96mg/kg,respectively.2.A new method for the simultaneous determination of puerarin,daidzin,daidzein and genistein in Puerariae Lobatae Radix was established by ultra-high performance liquid chromatography-ultraviolet detection technology(UPLC-DAD).The Puerariae Lobatae Radix samples were extracted with 70%ethylene glycol,and the extracts were purified with PSA sorbent,and the purification conditions were optimized.Under the optimized experimental conditions,puerarin,daidzin,daidzein and genistein had good linear correlations(R~2≥0.9981)in a certain concentration range,and the limits of detection and quantification were in the range of 0.020～0.086mg/L and 0.065～0.290 mg/L,respectively.The recovery rate of the above target analytes in Puerariae Lobatae Radix was 90.5%～109.6%,and the relative standard deviation was not higher than 7.7%.The established method was used to analyze the Puerariae Lobatae Radix from eleven regions in China,and the contents of puerarin,daidzin,daidzein and genistein in the target compounds were distributed in the range of 4.14～101.90 mg/g,0.53～14.90 mg/g,0.14～1.36 mg/g and 0.010～0.15 mg/g,with good effect.3.A new analytical method for the simultaneous determination of curcumin,dimethoxycurcumin and demethoxycurcumin in Curcumae Longae Rhizoma was established using ultra-performance liquid chromatography-ultraviolet detection technology(UPLC-DAD).Curcumae Longae Rhizoma was extracted with 50%acetonitrile and the extraction conditions are optimized.Under the optimized experimental conditions,curcumin,dimethoxycurcumin and demethoxycurcumin had good linear correlations(R~2≥0.9947)in the concentration ranges of 2～200 mg/L,1～100 mg/L and 1～100 mg/L,respectively,and the limits of detection and quantification were in the ranges of 0.0061～0.016 mg/L and 0.020～0.053 mg/L,respectively.The recovery rate of spiked was 91.9%～102.5%,and the relative standard deviation was less than 14.6%.The established method was applied to analyze Curcumae Longae Rhizoma from ten origins,and the measurement effect was good.The contents ofcurcumin,dimethoxycurcumin and demethoxycurcumin ranged from 2.67～22.23 mg/g,1.07～11.83 mg/g and 0.66～13.61 mg/g,respectively. |
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