Construction Of And Photosynthetic Hydrogen Production Of Chlorella-Escherichia Coli Co-aggregates Based On Biomimetic Silicification

Energy and environment are two global issues that human face in today’s development process.The massive use of fossil fuels by humans has caused serious climate problems.Developing and utilizing renewable energy and improving the energy structure have become an important component of global sustainable development strategies.As one of the most promising forms of clean and renewable energy,microalgae bioenergy has attracted increasing concern and attention.Microalgae hydrogen production is an ideal bioenergy.However,due to the oxygen sensitivity of microalgae hydrogenase,microalgae hydrogen production is still in the theoretical research stage.In the process of natural evolution,living organisms gradually form a mechanism of biomineralization.Through biomineralization,living organisms can synthesize inorganic nanomaterials to modify themselves to adapt to environmental changes.Inspired by biomineralization,researchers combine living organisms with materials to provide them with new functions.These biomimetic mineralized organisms have broad application prospects in the fields of bioenergy,environmental protection,biocatalysis,biotherapy,and other fields.Therefore,we envisage modifying the photosynthetic hydrogen production performance of microalgae through biomimetic mineralization.Previous modification of by biomimetic mineralization were all aimed at the transformation of a single species,while this paper realized construction of coaggregates of Chlorella pyrenoidosa and Escherichia Coli(E.coli)through biomimetic silicification.In the cell coaggregates,the photosynthesis of Chlorella provides oxygen and nutrition for Escherichia coli,while Escherichia coli consumes oxygen through respiration and produces acetic acid to promote the respiration of Chlorella,thus creating an anaerobic microenvironment to maintain the activity of hydrogen enzymes and promote photosynthetic hydrogen production.We further studied the effect of initial p H of the culture medium on photosynthetic hydrogen production of ChlorellaE.coli co-aggregates.