Study On The Separation Of α-Arbutin From Enzymatic Conversion Solution By Continuous Chromatography

α-Arbutin belongs to the glucoside category and is widely used as a whitening additive in cosmetics and pharmaceuticals.In industry,α-arbutin is mostly synthesized by biotransformation.After simple treatment,theα-arbutin enzymatic transformation solution obtained from sucrose also contains isomaltulose and hydroquinone,which need to be further separated and purified.Chromatography is a clean and efficient separation method,but research on its separation and purification ofα-arbutin is still incomplete.In this paper,the technical process of separatingα-arbutin,isomaltulose and hydroquinone with ligand exchange resin was discussed.A three zone simulated moving bed(SMB)device was designed and used for actual separation.The conclusions are as follows:Firstly,the resin media suitable for separating isomaltulose,arbutin,and hydroquinone were selected,and the separation capacities of adsorption resins and ligand exchange resins were compared.Moreover,the interaction force between the resin and the adsorbates was calculated.The results showed that the ligand exchange resin has better separation efficiency than the adsorption resin,and ZG SPC106-K sulfonic-acid resin has the best separation efficiency for the three components,the resolution is 1.52 and 1.12,respectively.It has been proved that the adsorption capacity of the resin for hydroquinone,α-arbutin,and isomaltulose decreases in turn,and the adsorption is affected by both coordination and hydrogen bonding.Secondly,the adsorption process of three components on ZG SPC106-K resin were studied from three aspects of adsorption isotherm,kinetics and thermodynamics.In addition,the chromatographic model and model parameters were determined.Results indicated that the M-Langmuir isotherm adsorption model can well fit the adsorption behavior of resin;ΔH<0,ΔS<0,andΔG<0,ZG SPC106-K resin can adsorb three components spontaneously;The pseudo-second-order kinetics model can better describe the adsorption kinetic behavior of components.The simulation effect of the mass transfer diffusion(TD)model is good,and the axial diffusion coefficient and the total mass transfer coefficient k _L are calculated by empirical formula method;Column temperature and the flow rate of eluent have a significant impact on the total mass transfer coefficient,as the column temperature increases,the k _L ofα-arbutin and hydroquinone increases,as the flow rate of elution increased,the k _L of the three components increases.Finally,an experimental study was conducted on the separation ofα-arbutin from enzymatic conversion solution using a three zone SMB.Under the conditions of 20 m L/min flow rate in Zone I,6.76 m L/min flow rate in Zone II,10.65 m L/min flow rate in Zone III,and a switching time of 14 minutes,the 2-2-2 configuration of three zone SMB can separate isomaltulose from the enzymatic conversion solution.Under the conditions of a flow rate of22 m L/min in Zone I,10.79 m L/min in Zone II,15.90 m L/min in Zone III,and a switching time of 10 minutes,the 2-2-2 configuration of a three zone SMB can separateα-arbutin and hydroquinone.Finally,the purity and recovery ofα-arbutin are 99.05%and 93.46%,respectively,the purity and recovery of isomaltulose were 97.78%and 98.33%,respectively,the purity and recovery of hydroquinone are 95.29%and 94.32%,respectively.