A Preliminary Study On The Effect Of Resistin Secreted By Adipose Tissue Lmo4 On Hepatic Inflammation And Gluconeogenesis

With the development of society,obesity has become one of the main factors threatening human health.Obesity leads to a variety of metabolic diseases,such as type2 diabetes,cardiovascular disease,and non-alcoholic fatty liver disease.At present,the strategy adopted by most obese people,especially young people,is dieting.However,dieting is difficult to maintain,and some people regain their weight after dieting,and then diet again,resulting in cyclical weight fluctuation(weight cycling).Weight cycling brings huge hidden dangers to human health.At present,there are few reports on the mechanism of weight cycling.Therefore,in-depth study of the mechanism of weight recovery in weight cycling and the mechanism of metabolic syndrome caused by weight cycling play an important role in the treatment of obesity.In the early stage,C57 BL / 6 mice were used as subjects.It was found that after the mice experienced weight cycling,their visceral adipose tissue was heavier and hepatic inflammation increased.Based on the previous results,this paper found that the expression of Lmo4 in adipose tissue was up-regulated in mice after weight cycling.Using an in vitro differentiation model of precursor adipocytes,it was found that knocking down / overexpressing Lmo4 in 3T3-L1 cells would inhibit / promote the proliferation and differentiation of precursor adipocytes.Further research found that Lmo4 can also promote the expression and secretion of resistin in adipose tissue,and resistin acts on the liver through circulation.In this paper,primary hepatocytes and C57 BL / 6 mice were used to demonstrate in vitro and in vivo that resistin can upregulate the expression of the rate-limiting enzymes Pck1 and G6 pc of the gluconeogenesis to upregulate the glucose levels in the liver and serum;On the one hand,resistin also can activate the STAT3 pathway,up-regulate the expression of proinflammatory factors Il6,Il8,etc.causes hepatic inflammation.In summary,this thesis studies that the Lmo4-Resistin signal axis can regulate the crosstalk between adipose tissue and liver tissue,which provides a theoretical basis for the mechanism of metabolic disorders caused by weight cycling.