The Study Of Laryngopharyngeal Microbiome In Laryngopharyngeal Reflux Diseases

Objective:1.To clarify the correlation between laryngopharyngeal reflux disease(LPRD)and microbiological changes in the laryngopharyngeal area.2.To clarify the correlation between the throat-related risk factors and the microbiological changes of the larynx.Methods:The patients with suspected LPRD in the Otorhinolaryngology Head and Neck Surgery Clinic were scored using the reflux symptom index(RSI)and the reflux finding score(RFS)and Risk factors,such as Smoking,drinking,irregular eating,associated with laryngopharyngeal disease were assessed,which would satisfy the RSI score>13 Patients with scores and/or RFS scores>7 were given laryngopharyngeal secretions while undergoing electronic nasopharyngoscopy,were enrolled in the suspected LPRD experimental group,and received proton pump inhibitors(PPI)treatment for at least 8 weeks,and those who are effective will eventually be included in the case group.Healthy volunteers were also recruited to collect laryngopharyngeal secretions specimens,and analyze the laryngopharyngeal microbiota by the second-generation high-throughput sequencing method.Statistical analysis was performed with SPSS 24.Results:This experiment finally included 23 cases in the case group and 8 cases in the healthy control group.The results showed that the laryngopharyngeal microbiota mainly includes Streptococcus(MRA=0.214251),Prevotella(MRA=0.116487),Neisseria(MRA=0.092210),Haemophilus(MRA=0.087854),actinomycetes Bacteria(MRA=0.065657).The average value of Chaol in the case group was 502.81±300.78,and the average value of the control group was 943.93±637.61,suggesting that the species diversity of the laryngopharyngeal flora of the case group was significantly lower than that of the control group(P<0.05);at the departmental level,the laryngopharynx of the case group The relative abundance of Prevotellaceae in the laryngopharyngeal flora was significantly higher than that of the control group(P<0.05);at the genus level,the relative abundance of Prevotella in the laryngopharyngeal flora of the case group The degree was significantly higher than that of the control group(P<0.05).A number of laryngopharyngeal-related risk factors are related to the laryngopharyngeal microbiota structure,among which drinking is an environmental factor that significantly affects the species richness and uniformity of the community.