Effect Of Coptis Plus Pinellia On Improving Diabetic Cognitive Dysfunction In Animals

Objective:Based on literature review and preliminary laboratory work,this topic explores the effects of Coptis-pinellia on glucose and lipid metabolism,inflammation and cognitive function in diabetic mice,as well as the effect of TCM syndromes,and is a diabetesrelated disease The research and development of drugs provides scientific theoretical basis.Methods:83 SPF-grade healthy male ICR mice were randomly divided into a blank group(n=20)and a model group(n=63).The mice in the model group were given high-fat and high-sugar diet combined with intraperitoneal injection of streptozotocin 100 Diabetes model was replicated at mg·kg-1;72 hours after the model was replicated,FBG was detected,and FBG≥11.1 mmol·L-1 mice were included in the experiment as a successful model;the diabetic mice were randomly divided into diabetes model groups(n=19),Coptidis-pinellia group(n=27),metformin group(n=14).The mice in the Coptidis-pinellia group and the metformin group were given intragastric administration every day for 4 consecutive weeks.FBG of diabetic mice was measured every week,and autonomous activity experiment and TCM syndrome evaluation experiment were carried out every week.After the administration,an oral glucose tolerance test was performed to determine AUC,and the fat coefficient and Lee’s index of diabetic mice were determined.Four weeks after the administration,a new object recognition experiment and a Morris water maze experiment were performed to evaluate the learning and memory ability of diabetic mice.After Morris water maze experiment,the levels of GSP,TC,TG,LDL-C and HDL-C in diabetic mice were measured;HE staining was used to observe the morphological changes of the hippocampus of each group of mice;enzyme-linked immunosorbent assay was used to determine diabetes Mouse IL-6 expression and INS content were calculated and HOMA-IR was calculated.Results:60 mouse models were successfully replicated,with a modelling rate of 95%.Compared with the blank group,the FBG of mice in the model group was significantly increased(P<0.01),and GSP had a tendency to increase;compared with the model group,the FBG of mice in the Coptidis-pinellia group and the metformin group was significantly decreased(P<0.01,P<0.05),GSP is significantly reduced.In the oral glucose tolerance test,compared with the blank group,the AUC of the model group was significantly increased(P<0.01,P<0.05);compared with the model group,the AUC of the Coptidis-pinellia group and the metformin group were significantly reduced(P<0.01,P<0.05).Compared with the blank group,the serum levels of TC and LDL-C in the model group were significantly increased(P<0.01);compared with the model group,the TC,TG,LDL-C and HDL-C in the Coptidis-pinellia group and the metformin group No significant changes.Compared with the blank group,the HOMA-IR of the model group was significantly increased(P<0.01);compared with the model group,the HOMA-IR of the Huanglian-Banxia group was significantly decreased(P<0.01).In the TCM syndrome experiment,compared with the blank group,the C value of red tongue and red nose in the model group increased significantly(P<0.01),and the number of autonomous activities and standing numbers decreased significantly(P<0.01);compared with the model group,The C value of the red tongue and red nose of the mice in the Coptidis-pinellia group and the metformin group was significantly reduced(P<0.01),and the number of independent activities in the second week of the metformin group was significantly increased(P<0.05)In the Morris water maze experiment,compared with the blank group,the model group’s positioning voyages 3rd,5th,8th and the test period’s escape latency increased significantly(P<0.05);compared with the model group,the metformin group’s 5th and test period The period of escape latency was significantly reduced(P<0.05).The escape latency of the Coptidis-pinellia group at the 5th time and the test period was significantly reduced(P<0.05);compared with the metformin group,the escape latency of the Coptidis-pinellia group at the 8th time was significantly reduced(P<0.05).There was no significant difference in the relative recognition index of the new object recognition experiment between the groups of rats(P>0.05).Compared with the blank group,the hippocampal neurons of the model group were loose and irregular,and the nucleus pyknosis occurred.Compared with the model group,the hippocampal neurons of the Coptidis-pinellia group and the metformin group were all loosened or apoptotic.Compared with the blank group,the IL-6 expression in the brain tissue of mice in the model group was significantly increased(P<0.05).Compared with the model group,the expression of IL-6 in the brain tissue of the mice in the Coptidis-pinellia group and the metformin group was significantly decreased(P<0.01,P<0.05).Conclusion:Rhizoma Rhizoma Pinelliae may reduce the expression of inflammatory factor IL-6 in the brain tissue of diabetic mice,thereby reducing the cognitive impairment of diabetic mice.Rhizoma Rhizoma Pinelliae can also improve the C value of red tongue red claw red in diabetic mice.Elevated TCM symptoms can also improve the glucose and lipid metabolism process in diabetic mice.