Correlation Of Polymorphisms In The Grb2 Locus Rs9907177 (C>T) And Hypertensive Disorders During Pregnancy

Objective: To explore the relationship between the polymorphism of rs9907177(C> T)in growth factor receptor-bound protein 2(Grb2)and Hypertensive disorders of pregnancy(HDP),and to provide experimental and theoretical basis for screening people with high risk group of clinical HDP.Methods: A total of 378 pregnant women in the obstetrics department of the Affiliated Hospital of Yanbian University were selected,including 190 cases of hypertensive disorder complicating pregnancy as the experimental group and 188 cases of healthy group without complications as the control group.Elital venous blood samples were collected from the study subjects.Regulome-DB prediction software was used to find the target gene Grb2,and then the representative single nucleotide polymorphisms(SNPs)were found.The whole blood DNAwas extracted by DNAextraction kit and the second-generation sequencing of Grb2 gene was carried out.Some blood samples were randomly selected to detect the accuracy of the second-generation sequencing of Grb2 gene by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP).The genotype distribution of Grb2 gene locus rs9907177 was analyzed by chi-square test.Binary Logistic regression model was used to analyze the odds ratio(OR)and 95%confidence interval(CI)between SNPand HDP.Results:(1)There were two alleles C and T at Grb2 rs9907177,andthree genotypes CC,CTand TT.The genotypic distribution frequencies of the subjects were analyzed by goodness of chi-square test,and the results showed that they met the requirements of Hardy-Weinberg genetic balance test(P>0.05).that is,the samples of 190 HDPexperimental groups and 188 healthy normal groups were representative.(2)Agarose gel electrophoresis results of Grb2 rs9907177 showed that TT genotype was a 123 bp fragment,CC genotype was 40 bp and 83 bp fragment,and CT genotype was 40 bp,83 bp and 123 bp fragment.(3)There was no significant difference in distribution frequency of genotypes between the experimental group andthe control group(P > 0.05).(4)In Grb2 rs9907177 locus,the CC genotype is wild type,the CT genotype is heterozygous type and the TT genotype is mutant type.Binary Logistic statistical analysis showed that compared with the CC genotype,the CT genotype is P=0.044,that is,P<0.05,which has statistical difference,and its corrected OR=1.628 and 95% CI = 1.400-1.988.That is,Grb2 rs9907177 CT genotype is the dangerous genotype of HDP.(5)The dominant model(TT/CT+CC)and the recessive model(CT+TT/CC)were analyzed by binary Logistic analysis.The results showed that in the recessive model,compared with Grb2 rs9907177 CC genotype,the risk of HDP in the combination of CT and TT was 1.554 times higher(corrected OR=1.554,95%CI=1.009-2.395,P=0.046).Conclusion:1.The single nucleotide polymorphism of Grb2 rs9907177(C>T)is correlated with HDP susceptibility.2.Genotypes carrying Grb2 rs9907177(C>T)CT and TT combination had a higher risk of HDPthan those carrying CC genotype.