Research On The Mechanism And Compatibility Of Guantong Prescription In The Prevention And Treatment Of Coronary Heart Disease And Angina Pectoris Based On Network Pharmacology

Objective:Based on the method of network pharmacology,to analyzes the compatibility law of Guantong prescription and the mechanism of preventing and treating coronary heart disease and angina pectoris,so as to provide reference for the clinical application of Guantong prescription.Methods:The main chemical components and targets of 8 drugs in Guantong Prescription were obtained through TCMSP、TCMID and DRUGBANK databases combined with literature.The active components of traditional Chinese medicine were screened according to ADME,and the gene symbol corresponding to the target was obtained from Uniprot database.Main targets of coronary heart disease angina pectoris were obtained by Gencards,OMIM,TTDdatabases.Venny online tool was used to obtain the intersection targets of coronary prescription and coronary heart disease angina pectoris.String platform was used to analyze protein interaction,construct PPI network map of protein interaction,and explore its potential core modules.Metascape platform was used to analyze the"drug-disease interaction target" and its biological processes and pathways,and then Cytoscape3.9.0 software was used to construct the "Guantong Prescription-coronary heart disease angina target-pathway network".Results:The core active components of Guantong Prescription for coronary heart disease and angina pectoris are quercetin,β-Sitosterol,stigmasterol and ginsenoside rh2 predict that the targets of Guantong formula in the treatment of coronary heart disease and angina pectoris are mainly AKT1,IL6,TNF,VEGFA,TP53,Jun and so on.The main pathways of these targets are lipid and atherosclerosis,tumor necrosis factor signaling pathway,insulin resistance,HIF-1 signaling pathway,diabetes cardiomyopathy,interaction of neuroactive ligand receptors,cell aging,cAMP signaling pathway,platelet activation and other pathways.These pathways are related to the metabolic processes of anti coronary atherosclerosis,regulating blood glucose,blood lipids,anti-inflammatory,improving myocardial hypoxia tolerance The mechanism of platelet inhibition is closely related.Conclusion:This study found that the main active components of Guantong prescription act on multiple different targets and play a therapeutic role on coronary heart disease and angina pectoris through multiple different pathways.This study preliminarily revealed the multi-component,multi-target and multi-channel mechanism of Guantong recipe in the treatment of coronary heart disease and angina pectoris,which provided a basis for the further study of Guantong recipe.