Clinical Observation On The Treatment Of Chronic Aplastic Anemia With Bushen Yisui Prescription And The Analysis Of Its Effect On Regulation Scheme Of T Cell Subsets And TNF-α

Objective:Through clinical research,to observe the change of TCM syndrome and Hemogram of the patient with chronic aplastic anemia,who are treated by Bushen Yisui prescription as the core clinical treatment,to determine the clinical efficacy and analyze its safety.This research also analyze ointment effect on regulation scheme of T cell subsets and TNF-αMethods:A retrospective study was conducted and 37 patients with chronic aplastic anemia were randomly seprated into observation group and control group.The observation group was given Cyclosporine capsule,Stanozolol Tablets and kidney tonifying cream.The observation group was given cyclosporine soft capsules,Stanozolol Tablets and Bushen Yisui prescription.The control group was treated with the same dose of Cyclosporine capsule and Stanozolol Tablets,without traditional Chinese medicine treatment.Evaluating the patients general data,which includes hemogram,TCM syndrome score,ECG,liver and kidney function and other safety indicators after 6months of treatment,then to analyse and determing the Clinical treatment.Meanwhile,collecting the patients peripheral blood and testing the changes of expression level of T lymphocyte subsets CD4 +,CD8 +,CD4 + / CD8 + and cytokine TNF-α before and after treatment.The data were analyzed by spss22.0 software. < 0.05 was used as the significant difference,which was statistically significant.Result:1.Bushen Yisui prescription has a significant effect in the treatment of chronic aplastic anemia,and the clinical effective rate is 84.21%2.Compared with the group before and after treatment,the score of TCM syndrome decreased significantly,and the level of hemogram increased.Compared with the control group,the observation group is better in the improvement of TCM syndromes and the level of leukocyte and hemoglobin.There was no significant difference in the incidence of adverse reactions between Bushen Yisui prescription and Western medicine.CD4 +,CD4 + / CD8 + in both two group increased,and the level of CD8 + decreased after the treatment;hence,the effect of the treatment group was better than that of the control group(P< 0.05).The result suggests that the use of Bushen Yisui prescription plays a role in regulating abnormal immunity.TNF-α in both groups decreased then the observation group was better than the control group,and the difference was statistically significant(P < 0.05).The result suggests that Bushen Yisui prescription treatment achieves the goal of controlling the level of negative hematopoietic factors and improving hematopoietic function.Conclusion:Bushen Yisui prescription can help the patients with chronic aplastic anemia and improve the TCM syndrome and hemogram.It has remarkable clinical effect.This prescription reduces the expression level of TNF-α and improve the hematopoietic function,adjust the abnormal immunity of patients.This study reckons that the unusal of T cell subsets may indirectly participated in the pathogenesis of aplastic anemia,and this untypical situation of T cell subsets offers a potential insight for future research and treatment.