Research Of The Gut Microbiota Diversity In Liver Transplantation Recipients During The Perioperative Period Based On 16s RRNA Sequencing

Objective:1.Detect the gut microbiota of liver transplantation patients and healthy people by 16 s rRNA sequencing.2.Compare the gut microbiota of liver transplantation patients with healthy people at the levels of phylum,family and genus.3.Evaluate the effect of liver transplantation on intestinal microecological disorder.Methods:The gut fecal microbial community signatures of 37 Chinese adults were studied for 69 fecal samples.Stool samples were collected from the First Hospital of Shanxi Medical University in 2020-2021.The entire study design and procedures involved were established following the Declaration of Hesinki.Writer infotmed consent forms were signed before the time of sample collection.The Ethics Committee of the First Hospital of Shanxi Medical University approved the protocols.We excluded participants suffering from any symptoms of constipation,bloody stool,diarrhea,or other gastrointestinal disease and those who were administered antibiotics(oral or injectable)in the previous three months.All stool samples were collected within 4 h.stool samples were collected in sterile containers provided to the volunteers and were stored at-80℃.Sampling was performed using all standard protocols and regulations.Our analysis was conducted on a total of 69 fecal samples subdivided as follows: before liver transplantation(BLT,16 samples);liver transplantation 1 week(LT1W,16 samples);liver transplantation 2 weeks(LT2W,16 samples)and control group(CG,21samples).Results:The healthy gut microbiota predominantly consisted of the phyla Firmicutes and Bacteroidestes,following by Proteobacteria and Actinobacteria.Compared with healthy people,dur to the dominant bacteria in patients with chronic liver disease losing their advantages in the gut,the antagonistic effect on the inferior bacteria was reduced.The inferior bacteria multiplied in large numbers duting this peocess.Some od these significant changes were observed in bacterial species blonging to Enterococcus,Klebisiella,and Enterobacter,which increased in patients’ intestines.There were low abundances of signature genes such as Bacteroides,Prevotella,and Ruminococcus.Blautia and Bifidobacteriun(considered probiotics)almost disappeared after liver transplantation.Conclusion:There is an altered microbial composition in liver transplantation patients and a distinct signature of microbiota associated with the perioperative period.