Study On Untargeted Metabolomics And Antioxidant Activity Of Eucommia Ulmoides Oliver

Eucommia ulmoides Oliver(EU)contains iridoid terpenoids,lignans,flavonoids,steroids and other medicinal components and natural Eucommia rubber,which has high value in the fields of medicine and industry,but the comprehensive analysis of different tissue metabolites and rubber of EU is still very limited.In this study,we analyzed the components of leaves,seeds and barks of EU by using UHPLC-Q-TOF MS untargeted metabolomics before2373 metabolites were identified in total.Through the combination of Principal Component Analysis(PCA)、 one-way Analysis of Variance(ANOVA)and Partial Least Squares Discriminant Analysis(PLS-DA),116 significantly different metabolites were screened(VIP > 1.5,P < 0.05).In the process of cluster analysis and correlation analysis of differential metabolites,the differences in expression patterns of metabolites in different tissues and the mutual regulation of metabolites were further revealed.In order to explore the significant pathways formed by difference of the tissues in EU,we performed KEGG pathway analysis.In this way,it found that biosynthesis of phenyl propanoids,biosynthesis of alkaloids derived from histidine and purine,ABC transporters,biosynthesis of plant hormones and phenylalanine metabolism were the main pathways that made metabolic difference in various tissues of EU(p<0.001).At the same time,the rubber contents of EU leaves,trunk bark,branch bark and seeds were quantitatively analyzed by pyrolysis gas chromatography(Py-GC).It was found that the rubber contents of EU seeds and trunk bark were the highest,while that of leaves were the lowest.Finally,the antioxidant activities of EU leaves and seeds were evaluated by hydroxyl radical scavenging method,superoxide anion method,DPPH method,ABTS method and FRAP method.This work revealed the characteristics and laws of metabonomics in different tissues of Eucommia ulmoides for the first time,which provided a theoretical basis for the development of new medicinal components and the biosynthesis pathway,and laid a foundation for the basic research of pharmacodynamics and the development of targeting function of Eucommia ulmoides.