The Research Of Degradation Process Of Bioactive Glass Jawbone Repair Scaffold Containing Phosphorus

Objective To study the effects of phosphorus replacing silicon and CaCl₂ replacing Ca O on mechanical performance and biological activity of porous bioactive glass（BG）scaffolds,and aims to discover the factors that affect the degradation and mineralization properties of chlorine-containing bioactive glass bone repair scaffolds,and provide technical support for regulating the coordination of mineralization and degradation of biomaterials,,and also provide experimental basis for the subsequent research and development of animal in vivo experiments and cell culture and related products.Methods Based on the ZnO nitrogenous silicate glass studied in the previous research group,P₂O₅ was used to replace Si O₂ and the basic glass was prepared by melting method.The porous BG scaffold containing phosphorus was maked by the organic foam impregnation process.A set of scaffolds with the best mineralization activity was prepared,and then CaCl₂ was used to replace Ca O.The curved strength（BS）,compressive strength（CS）,degradation performance and mineralization activity were measured.BS is measured by three-point bending experiment,CS is measured by uniaxial compression experiment.The degradation performance was characterized by the weight loss ratio of porous BG scaffolds immersion experiment.The mineralization activity was characterized by X-ray diffraction（XRD）and Scanning Electron Microscope（SEM）analysis of the surface image of the porous BG scaffold and observation of the surface morphology.Results（1）When SiO₂ was replaced with P₂O₅,the CS and BS of groups A B and C gradually increased and the difference in statistical analysis was statistically significant（P<0.05）,after pairwise comparison,the results showed that there were differences among the three comparison groups（all P<0.05）;After 7 days immersion in simulated body fluid,the weight loss ratio of group A,B and C gradually increased gradually and the difference in statistical analysis was statistically significant（P<0.05）,after pairwise comparison,the results showed that there were differences among the three comparison groups（all P<0.05）;After immersion in simulated body fluid for 7 days,group A had no mineralization activity,and group C had stronger mineralization activity than group B.（2）When Ca O was replaced with CaCl₂,the CS and BS of C,C1,C2 and C3 groups decreased gradually and the difference in statistical analysis was statistically significant（P<0.05）,after pairwise comparison,the results showed that there were differences among the six comparison groups（all P<0.05）;The weight loss ratio of group C,C1,C2and C3 gradually increased after immersion in simulated body fluid for 24 hours,and the difference in statistical analysis was statistically significant（P<0.05）,after pairwise comparison,the results showed that there were differences among the six comparison groups（all P<0.05）;After immersion in simulated body fluid for 24 hours,the four groups C,C1,C2,and C3 all had mineralization activity,and it gradually increased.Conclusion（1）P₂O₅can improve the mechanical properties and biological activity of the porous BG scaffolds,but when the content of P₂O₅was 15wt%,the porous BG scaffold collapsed,when the content of P₂O₅was 10wt%,the mechanical properties and biological activity of porous BG scaffolds were the strongest;（2）CaCl₂ can reduce the mechanical properties of the porous BG scaffolds,and its mass percentage should be controlled below 12wt%;In addition,it can also improve the biological activity of the porous BG scaffolds.