| Objective : Through experiments to explore the effects of transcranial direct current stimulation(t DCS)on neurological function,cerebral blood flow and neovascularization in rats with cerebral infarction,and to further clarify the effects of t DCS on neovascularization-related factors VEGF and CD34 in rats with cerebral infarction,so as to provide a theoretical basis for the clinical treatment principle of t DCS.Method: Twenty-eight adult SPF male SD rats were selected and randomly divided into a sham operation group(sham),a model group(model)and a transcranial direct current stimulation group(t DCS),of which 10 were in the model group and 10 in the t DCS group,and 8 in the sham group.The middle cerebral artery occlusion model was established in the model group and t DCS group,and the length of the suture in the sham group was controlled at6-10 mm.Rats in the t DCS group received anodal stimulation with a stimulation parameter of200 u A,20min/d.Electrodes were installed in both the model group and the sham group,but no current stimulation was applied.(1)Using the longa neurobehavioral scoring method,the rats in each group were scored for neurobehavioral before modeling,24 hours after modeling,and 12 days after modeling;(2)3D ASL was used to observe the changes of cerebral blood flow in the model group and t DCS group at 3d and 12 d after modeling;(3)One rat in the model group and t DCS group was randomly selected 24 h after modeling and 12 d after modeling,a total of 4 rats,and the changes in the number of cerebral neovascularization were observed by tissue immunofluorescence method;(4)Western-blot method was used to detect the expression of VEGF and CD34 protein in model group and t DCS group at 24 h and 12 d after modeling.Results:(1)The neurobehavioral scores of the rats in each group: the rats in the sham group had a neurobehavioral score of 0 before modeling,24 hours after modeling,and 12 days after modeling;There was no significant difference in the neurobehavioral scores of the rats in the t DCS group(P>0.05),and 12 days after modeling,the neurobehavioral scores in the t DCS group were lower than those in the model group(P<0.05);(2)Observation of cerebral blood flow by 3D ASL: 3 days after modeling,there was no significant difference in cerebral blood flow between model group and t DCS group(P>0.05).The increased flow was statistically significant(P<0.05);(3)Observation of cerebral neovascularization by immunofluorescence:12 days after modeling,the number of new blood vessels in the model group and t DCS group increased significantly compared with 24 hours after modeling.After 12 days,the number of new blood vessels in the t DCS group increased compared with the model group.The sample size of this study was small and not statistically significant;(4)Western-blot assay to detect the expression of VEGF and CD34: 12 days after modeling,the protein expression of VEGF was significantly increased compared with the model group and the sham group(P<0.01),and the t DCS group was significantly increased compared with the model group.High(P<0.001);the protein expression of CD34 in the model group was higher than that in the sham group(P<0.05),and the expression in the t DCS group was significantly higher than that in the model group(P<0.001).Conclusions:(1)t DCS can reduce the neurobehavioral score and improve the symptoms of neurological deficit in rats with cerebral infarction;(2)t DCS can promote cerebral infarction The recovery of cerebral blood flow in rats and the improvement of blood perfusion;(3)t DCS can promote the regeneration of new blood vessels in rats with cerebral infarction,but this conclusion needs further verification;(4)t DCS can increase the expression of VEGF and CD34 in the brain tissue of rats with cerebral infarction,thereby increasing the number of new blood vessels to establish collateral circulation,and promote cerebral infarction rats recovery. |
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