Study On The Evaluation Method Of Chelating Agent For Removing Beryllium

Beryllium has excellent nuclear and physical properties.It is an indispensable material component in many fields,such as nuclear energy,national defense,aerospace,and electronic industry.Although beryllium and its compounds are "strategic" engineering materials,they are also carcinogenic to humans and are classified as Group 1 by International Agency for Research on Cancer(IARC)in2017.Moreover,beryllium fluoride is the most toxic soluble beryllium salt.This brings serious safety hazards to the staff engaged in beryllium-related occupations.Therefore,the methods for preventing and treating beryllium disease become particularly important.Chelation therapy is one of the effective means to prevent and treat beryllium poisoning.Its critical problem is the selection of beryllium chelating agents.However,because of the dynamic and complex relationships in biological systems,chemicals that often qualify as ideal chelators in vitro may not demonstrate this in vivo,so finding an experiment method about the beryllium chelator can link in vivo and in vitro experiments is essential.This subject wants to establish a method for evaluating the effect of the beryllium chelating agent.This method can quickly,intuitively,and efficiently screen out chelating agents that can effectively chelate beryllium in vitro and verify the chelating effect on beryllium under physiological conditions and further explore the possible side effects of chelating agents.This paper will select beryllium fluoride as beryllium reagent,Ethylenediaminetetraacetic acid trisodium salt(trisodium EDTA),potassium sodium tartrate,and taurine as beryllium chelating agents.The first part of the experiment will use UV-Vis spectrophotometry to study the chelating effect of chelating agents on beryllium.In the experiment,chromazurine S was used as a chromogenic agent,and the chelation efficiency of beryllium was reflected by the degree of inhibition of the absorbance of chelated beryllium-chromazurine S by different chelating agents.The experimental results show that the chelating efficiency of three chelating agents to beryllium is: trisodium EDTA> potassium sodium tartrate> taurine.This method can be used as a general screening method for beryllium chelators.The second part of the experiment is to verify the chelating effect of the chelating agent on beryllium under physiological conditions.The experiment used serum as the matrix environment to simulate physiological conditions.Beryllium fluoride(0.2 μg Be/m L.serum)and chelating agent were added to the serum,and the beryllium-containing serum with different chelating agents was centrifuged using an ultrafiltration centrifuge tube.Finally,the content of beryllium ions in the serum concentrate and centrifugal fluid after centrifugation was determined by the inductively coupled plasma mass spectrometer(ICP-MS),which reflected the removal effect agent on beryllium in serum.After verifying the chelating effect of the chelating agent on beryllium under physiological conditions,it is also necessary to verify whether the chelating agent will have a side impact on the body or affect people’s other elements in the body.The samples were analyzed by ICP-MS.The results showed that trisodium EDTA would significantly impact Mg,Zn,Sr,Ba,Fe,and Cu elements in the body.In contrast,the high concentration of potassium sodium tartrate only influenced Mg and Ba elements.Summarize the results of three experiments reflecting that trisodium EDTA has a better chelating effect on beryllium but significantly impacts various metal ions in the body.Therefore,trisodium EDTA is not suitable for long-term use in the body but is more suitable as a treatment for acute beryllium disease.Therefore,potassium sodium tartrate is more suitable as a drug for preventing beryllium disease and treating chronic beryllium disease.At the same time,the experimental results also showed that Mg and Ba in the body seriously interfered with the removal effect of the chelating agent on beryllium.The research method established in this subject can not only quickly screen and compare chelators on beryllium in vitro environment but also simulate the internal environment of living organisms with the help of serum to verify the applicability of chelating agents to living organisms.This research method is not only applicable to the study of chelators on beryllium but also to the study of chelators on other toxic metals.The establishment of this research method has particular significance for preventing and treating toxic metal diseases.