Correlation Study Of Dynamic Contrast-enhanced Perfusion MR Imaging With IDH1 Genogype And ANXA1 Expression In Glioma

Purpose:This study aims to investigate the differences of quantitative parameters of dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)in different IDH1 types of glioma,and the corresponding diagnostic efficacy.To evaluate the correlation between quantitative parameters and ANXA1 expression.Methods:This study retrospectively analyzed 89 patients with glioma confirmed by surgery and pathology who visited Shandong Provincial Hospital from January 2019 to December 2021.The preoperative clinical and imaging data of glioma were analyzed.Siemens postprocessing workstation syngo was used to post-process the original DCE-MRI image,and the region of interest(ROI)was drawn in the tumor parenchyma area to obtain the following quantitative parameters:volume transfer constant(Ktrans),rate constant(Kep),and volume fraction of extravascular extracellular space(Ve).SPSS was used to statistically analyze the quantitative parameters of glioma with different IDH1 classifications,and ROC curve was used to evaluate the diagnostic efficacy of the quantitative parameters for different IDH1 glioma types.Collect the protein expression data of glioma with different IDH1 subtypes in the Tumor Genome Map Database(TCGA),and analyze the difference of ANXA1 expression in glioma with different IDH1 subtypes.Immunohistochemical staining was used to verify the difference of ANXA1 expression.The quantitative parameters were correlated with the expression of ANXA1.Spearman test was used to analyze the correlation between the quantitative parameters and the expression of ANXA1.Results:1.Compared with IDH1 mutant glioma group,IDH1 wild-type glioma has higher Ktrans and Ve values.The difference of Ktrans and Ve values of DCE-MR1 quantitative parameters among different IDH1 glioma groups was statistically significant.2.ROC curve analysis:when the Ktrans cut-off value is 0.034 min-1,the sensitivity,specificity and AUC of IDH1 classification are 0.843,0.846 and 0.831(0.729-0.933)respectively.When Ve cut-off value is 0.022,the sensitivity,specificity and AUC of IDH1 typing prediction are 0.947,0.538 and 0.659(0.523-0.795)respectively.Ktrans is the best quantitative parameter for differentiating different IDH1 types of gliomas.3.Kaplan-Merier survival analysis showed that the survival time of the group with high expression of ANXA1 was shorter than that of the group with low expression of ANXA1.In gliomas,the high expression of ANXA1 indicates poor prognosis.4.TCGA database and immunohistochemical staining showed that the expression of ANXA1 in IDH1 mutant glioma was lower than that in IDH1 wild glioma.The differences of ANXA1 in different IDH1 types of glioma tissues were statistically significant.5.In gliomas,the Ktrans value was positively correlated with the expression of ANXA1(r=0.774,p<0.001).Excluding IDH1 mutant gliomas,the correlation between Ktrans and ANXA1 expression was higher.(r=0.920,p<0.001).In gliomas,the value of Ve was positively correlated with the expression of ANXA1(r=0.428,p<0.001).Excluding IDH1 mutant gliomas,the correlation between Ve value and ANXA1 expression was higher(r=0.652,p<0.001).In IDH1 wild-type gliomas,the quantitative parameters of DCE-MRI,Ktrans and Ve,were positively correlated with the expression of ANXA1.Conclusion:DCE-MRI quantitative parameters have a certain reference value in distinguishing different IDH1 types of gliomas,and have a certain correlation with the expression of ANXA1.ANXA1 is highly expressed in IDH1 wild type gliomas and indicates a poor prognosis.