The Expression And Clinical Significance Of CXCL10 And CXCL16 In Serum And Placenta Of Patients With Intrahepatic Cholestasis Of Pregnancy

Objectives:The expression of chemokine CXC ligand 10(CXCL10)and chemokine CXC ligand 16(CXCL 16)in serum and placenta of patients with intrahepatic cholestasis of pregnancy(ICP)was examined to explore the relationship between the expression of these two chemokines and the pathogenesis and severity of ICP.In addition,we analyzed the correlation between these two chemokines and the adverse pregnancy outcomes,such as premature delivery,fetal distress,etc.and then provided a theoretical basis for early detection and intervention of adverse pregnancy outcomes in patients with ICP.Methods:64 pregnant women were selected in the study,20 normal pregnant women as control group,44 patients with intrahepatic cholestasis of pregnancy as case group,including 25 patients with mild intrahepatic cholestasis of pregnancy and 19 patients with severe intrahepatic cholestasis of pregnancy.The clinical data,mode of delivery and pregnancy outcome were collected.The expression of CXCL 10 and CXCL 16 in serum and placenta were compared among the three groups.The serum,placental tissue,clinical data,delivery mode and pregnancy outcomes were collected.The expression of CXCL 10 and CXCL 16 in serum and placenta tissue were compared among the three groups.Spearman and Pearson correlation analysis methods were used to study the correlation between the expression of CXCL 10,CXCL 16 and pregnancy outcomes.Results:(1)The expression of CXCL 10 and CXCL 16 in ICP was significantly higher than that of control group by ELISA(P<0.05).CXCL10 and CXCL16 may be related to the occurrence and development of ICP.(2)In the results of this experiment,CXCL 10 was expressed in the placenta tissues of the control group and the case group.The expression of CXCL 10 in placental tissue of ICP was higher than that of normal pregnancy group,and the difference was statistically significant(P<0.05).The expression of CXCL10 in placental tissue of severe ICP was higher than that of control group and mild ICP group,and the difference was statistically significant(P<0.05).The expression of CXCL 10 in placental tissue of mild ICP and control group was not statistically significant(P>0.05).(3)The expression level of CXCL16 in placental tissue of patients with ICP was higher than that of control group,and the difference was statistically significant(P<0.05).The expression level of CXCL16 in placental tissue of severe ICP was higher than that of control group and mild group,and the difference was statistically significant(P<0.05).The expression level of CXCL10 in placental tissue between patients with mild ICP and control group has no statistically significance(P>0.05).(4)Compared with the control group,the gestational age of delivery in the case group was earlier,the birth weight of the newborn was lighter,and the length of the newborn was smaller.There was also a statistical difference between severe ICP and mild ICP(P<0.05).The incidence of cesarean section,premature delivery,meconium-stained amniotic fluid,small for gestational age or low birth weight infants,fetal distress and Apgar score<7 in the case group were higher than those in the control group.The incidence of cesarean section,premature delivery and Apgar score<7 in severe ICP were higher than those in the control group,and the difference was statistically significant(P<0.05).There was no significant difference in the incidence of postpartum hemorrhage between the two groups(P>0.05).(5)The serum CXCL10 level was negatively correlated with gestational age,and positively correlated with cesarean section,premature delivery and Apgar score<7(P<0.05).Serum CXCL16 level was negatively correlated with gestational age,birth weight and birth length,and positively correlated with cesarean section rate and premature delivery rate(P<0.05).Serum total bile acid level was negatively correlated with gestational age,birth weight and birth length,and positively correlated with premature delivery rate,cesarean section rate(P<0.05).Conclusions:(1)The expression levels of CXCL10 and CXCL16 in serum and placental tissue of patients with ICP were significantly higher than those of normal pregnant women,and were positively correlated with the severity of the disease.The results showed that CXCL10 and CXCL16 may be related to the occurrence and development of ICP.(2)The levels of CXCL10 and CXCL16 in patients with ICP may be positively correlated with the incidence of adverse pregnancy outcomes.The possibility of adverse outcomes can be predicted in combination with other clinical data of patients during clinical diagnosis and treatment,so that intervention measures can be taken as soon as possible to improve maternal and fetal prognosis.