Study On The Clinical Effect Of Colquhounia Root Tablets On Damp Heat Stasis Obstruction Diabetes Nephropathy And The Protective Effect On Injured Podocyte

Objective:To evaluate the clinical efficacy of Colquhounia Root Tablets in on diabetes nephropathy by observing the changes of relevant laboratory indicators in patients with G1-3a A1-3 stage of damp heat stasis obstruction of diabetes nephropathy.On this basis,the damage of podocytes induced by high glucose conditions and the protective effect of Colquhounia Root Tablets on podocytes were determined through cell experiments.To provide more powerful clinical and experimental basis for clinical application of Colquhounia Root Tablets in the treatment of diabetes nephropathy.Methods:1.Clinical trial: A total of 72 patients who met the inclusion criteria were collected and randomly divided into test group and control group with 36 cases each.Both groups of subjects were treated with basic western medicine,and the test group was added with Colquhounia Root Tablets.The course of treatment is 12 weeks.Compare the changes of TCM symptoms,Urine protein/Creatinine ratio(UACR),Glomerular Filtration Rate(e GFR),Blood Creatinine(Scr),Blood Urea nitrogen(BUN),Glycosylated Hemoglobin(Hb A1c)and other relevant laboratory indicators of the two groups before and after treatment.And carry out statistical processing and comparative analysis.2.Cell experiment: Mouse podophyte cells 5(MPC5)were cultured in vitro.The experiment was divided into normal control group,model group and Colquhounia Root Tablets group(25μg/m L,50μg/m L).MTT method and flow cytometry were used to detect the cell activity and apoptosis rate,and Western blot was used to detect the protein expression levels of Podocyte specific protein Nephrin and apoptosis related factors Bax,Bcl-2.To clarify the protective effect of Colquhounia Root Tablets on podocyte apoptosis induced by high glucose.Results:1.Clinical trial results: After treatment,the TCM symptom score of patients in both groups decreased(P<0.01).And the reduction in the test group is more obvious.The total effective rate of the treatment group and the control group were 78.79% and 50.00% respectively,and the test group was better than the control group(P<0.01).UACR,SCR,BUN and e GFR in the two groups were improved,and the improvement in the test group was more obvious than that in the control group(P<0.05).There was no significant difference between the two groups in blood lipid and glucose indexes TG,TC,FBG and Hb A1 c before and after treatment(P>0.05),which were controlled within a reasonable range.2.Cell experiment results:(1)cell viability and apoptosis rate:The activity of cells decreased significantly and the apoptosis rate increased after high glucose treatment(P<0.01).After the treatment with different doses of Colquhounia Root Tablets,the cell viability of each group increased to different degrees(P< 0.01),and the cell apoptosis rate decreased(P<0.01);The improvement of high dose group was significantly better than that of low dose group(P<0.01).(2)Podocyte skeleton protein Nephrin: The expression level of the model group was the lowest,and the difference was statistically significant compared with the normal control group(P<0.01);Compared with the model group,the expression of the two dose groups of Colquhounia Root Tablets was up-regulated to different degrees(P<0.01),and the up-regulation of the high dose group was more significant(P<0.05).(3)Anti-apoptotic factor Bcl-2、Pro-apoptotic factor Bax: After high glucose stimulation,the expression of Bcl-2 decreased significantly(P<0.05),and the expression of Bax increased significantly(P<0.05),However,the intervention of different doses of Colquhounia Root Tablets reversed this situation to varying degrees.Conclusion:1.Colquhounia Root Tablets can improve the clinical symptoms and renal function of patients with G1-3a A1-3 stage of damp heat stasis obstruction of diabetes nephropathy,reduce urinary protein excretion,and delay disease progression;2.In this experiment,the podocyte injury model was established by high glucose induction,and the injury of podocyte under high glucose condition was clarified;3.Colquhounia Root Tablets can restore the expression of key protein Nephrin and protect damaged podocytes in high glucose environment by affecting podocyte proliferation and apoptosis and other cell biological behaviors.