Based On NF-κB Signal Pathway To Explore The Therapeutic Effect Of Sanhuang Xiexin Decoction On Inflammatory Injury Of Brain Tissue In Rats With Hepatic Encephalopathy

Objective: By establishing a rat model of hepatic encephalopathy(HE)and conducting relevant tests,according to the experimental results,based on NF-κB signal pathway studies the therapeutic effect and molecular mechanism of Sanhuang Xiexin Decoction on inflammatory injury of brain tissue in rats.RESULTS: After gavage administration of SHXXD at various concentrations,HE rats in each herbal treatment group gradually regained their appetite,decreased hair loss,drowsiness and coma compared with the model group,and improved their water maze performance(p<0.05).Pathological sections of liver: the rats in the model group showed severe liver tissue hyperplasia,blurred structure of liver lobules,disorganized cell arrangement,degeneration and necrosis,and inflammatory cell infiltration;compared with the model group,the degree of degeneration and necrosis,inflammatory cell infiltration,and tissue hyperplasia of liver cells in the lactulose group and SHXXD low and high concentration groups were all improved to different degrees,among which the improvement was more obvious in the SHXXD high concentration group.The blood levels of ammonia,ghrelin,ghrelin and albumin in the model group increased significantly compared with the blank group(p<0.05),and the albumin levels in the model group decreased significantly compared with the blank group(p<0.05);the blood levels of ammonia,ghrelin and ghrelin in the lactulose,SHXXD low concentration and high concentration groups increased significantly compared with the blank group(p<0.05);the blood levels of ammonia,ghrelin and ghrelin in the lactulose,SHXXD low concentration and high concentration groups increased significantly compared with the blank group(p<0.05).The levels of blood ammonia,glutamic transaminase and glutamic oxalacetic transaminase in the lactulose,SHXXD low concentration and high concentration groups all decreased to different degrees compared with the model group(p<0.05),and the levels of albumin in the lactulose,SHXXD low concentration and high concentration groups all increased to different degrees compared with the model group(p<0.05),with the changes in the lactulose and SHXXD high concentration groups being more obvious.The levels of tumor necrosis factor α,interleukin 1β and interleukin 6 in the rat serum inflammatory factors showed that the levels of tumor necrosis factor α,interleukin 1β and interleukin 6 in the model group increased significantly compared to the blank group(p < 0.05);the levels of tumor necrosis factor α,interleukin 1β and interleukin 6 in the lactulose group,SHXXD low concentration group and high concentration group all decreased to different degrees(p < 0.05).p<0.05),with significant decreases in the levels of inflammatory factors in the lactulose and SHXXD high concentration groups.The intestinal p H of the rats showed a significant increase in the model group compared to the blank group(p<0.05);the intestinal p H of the lactulose,SHXXD low concentration and high concentration groups all showed different degrees of decrease compared to the model group(p<0.05),with the lactulose and SHXXD high concentration groups converging to the normal group.The calculation of brain mass and water content in brain tissue showed that: compared with the blank group,brain mass and water content in the model group increased(p<0.05);compared with the model group,brain mass and water content in all treatment groups decreased to different degrees(p<0.05),among which the lactulose group and SHXXD high concentration group were more obvious.The results of real-time fluorescence quantitative polymerase chain reaction assay for inflammatory factors in rat brain tissues showed that the m RNA expression of tumor necrosis factor α,interleukin 1β and interleukin 6 in rat brain tissues increased significantly in the model group compared with the blank group(p<0.05);the m RNA expression of tumor necrosis factor α,interleukin 1β and interleukin 6 in rat brain tissues increased significantly in the lactulose group,SHXXD low concentration group and high concentration group compared with the model group(p<0.05).The m RNA expression of tumor necrosis factor α,interleukin 1β and interleukin 6 in rat brain tissues decreased to different degrees in the lactulose group,SHXXD low concentration group and SHXXD high concentration group compared with the model group(p<0.05).The results showed that the expression of p65 protein subunit and its phosphorylated protein in the NF-κB pathway in the rat brain was significantly increased in the model group compared with the normal group(p<0.05).The expression of the p65 protein subunit and its phosphorylated protein in the NF-κB pathway in the rat brain of the lactose,SHXXD low and high concentration groups decreased to different degrees compared with the model group(p<0.05),with the lactose and SHXXD high concentration groups significantly decreasing.Conclusions: Sanhuangxiexin Tang can reduce brain edema and inhibit the m RNA expression of TNF-α,IL-1β and IL-6 in the brain tissues of HE rats by improving their liver function,reducing the blood ammonia level and inflammatory cytokines such as TNF-α,IL-1β and IL-6 in the serum,regulating the intestinal p H of HE rats,and inhibiting the m RNA expression of TNF-α,IL-1β and IL-6 in the brain tissues of HE rats,thus inhibiting the phosphorylation of NF-κB pathway in the brain tissues.