Effects Of Long Non-coding RNA LINC00978 On Proliferation And Migration Of Thyroid Papillary Cancer Cells Through MAPK Signaling Pathway

Objectives To investigate the effect of Lnc RNA LINC00978 on the proliferation and invasion of papillary thyroid carcinoma and its possible mechanism.Methods 1 The papillary thyroid carcinoma TPC-1 cell line was selected for research,and when it was normalized culture to the logarithmic growth phase,it was transfected with different concentrations of gradient fluorescently labeled si RNA-CY3,and the transfection efficiency was observed 6 hours after transfection to determine the optimal transfected gene concentration;2 q RT-PCR to detect whether the construction of interfering LINC00978 cells was successful;3 The CCK-8 method was used to detect the cell proliferation ability.By observing the absorbance at 450 nm in each group,the detection was carried out at 24 h,48h,72 h,and 96 h after transfection,respectively;4 The scratch healing experiment was used to detect the cell migration ability.By comparing the changes in the cell migration area in each group simultaneously,the observation index was the 48 h cell migration rate;5 the Transwell experiment was used to detect the cell invasion ability.The number of cells penetrated to the back of the chamber,and the observation index is the number of invasive cells;6 The expression levels of signaling pathway-related proteins were detected by western blotting.Results 1 Lipofectamine 2000 can successfully transfect si RNA-CY3 into cells.When the gene concentration is 50 n M,the transfection effect is the best,and the transfection efficiency can reach more than 60%.2 The results of q RT-PCR showed that LINC00978 could be successfully inhibited by all three suppressor genes(P<0.01).The transfected siLINC00978-3,si-NC,and PBS were used as the transfection,control,and blank groups,respectively.3 The results of the CCK-8 experiment showed that: compared with the control group and the blank group,the cell proliferation ability of the transfection group was significantly reduced at 24 h,48h,72 h,and 96 h after transfection,and the differences were statistically significant(P<0.01).4 The scratch healing experiment showed that the cell migration rate of the transfection group at 48 h was significantly lower than that of the other two groups(P<0.01).5 The results of the Transwell invasion assay showed that compared with the control group and blank group,the invasion ability of the transfection group was weakened(P<0.01).6 The results of western blot showed that the expression of p-ERK1/2 protein in the transfection group was lower than that of the other two groups,and there was no significant difference in the expression of ERK1/2 protein among the groups.Conclusions Lnc RNA LINC00978 can promote papillary thyroid carcinoma’s proliferation,migration,and invasion.Its mechanism may be related to the regulation of phosphorylation of crucial molecules ERK1/2 in the MAPK signaling pathway.Figure 11;Table 10;Reference 123...