Study On The Anti-fatigue Effect Of The Crude Polysaccharide Of Floccularia Luteovirens In Mice

Fatigue refers to a state in which exercise capacity and body function are temporarily decreased.Modem medicine shows that long-term fatigue will cause a series of physiological problems and diseases.Therefore,research related to fatigue resistance has attracted much attention since this year.At present,the research on anti-fatigue focuses on relieving fatigue through nutritional intervention,in which polysaccharides,a natural active component,have good anti-fatigue and antioxidant effects and can help the collective to quickly restore the loss of sugars under fatigue.Floccularia luteovirens is also known as Armillaria luteo-virensins,is a kind of fungus with rich nutritional composition,high nutritional value and excellent economic effect.The crude polysaccharide of Floccularia luteovirens has antiinfluenza,immunomodulatory,antioxidant and antitumor effects,but its anti-fatigue effects are still unclear.In order to better investigate the antifatigue effects of crude polysaccharide of Floccularia luteovirens on mice,Mice models were as research subjects.Establishing the exercise fatigue mouse model to provide a theoretical basis for the crude polysaccharide of Floccularia luteovirens as an effective component of anti-fatigue medicine.As follows:In this study,40 C57BL/6J mice were stochastic divided into 5 groups,that is,blank control group,model group,model group combined with drug(low,medium,high dose)group.Firstly,the mouse faigue modle was established.Then observing and recording the mice were exposed recording the rotarod time and water rest time in fatigue rotarod experiment and forced swimming experiment.The anti-fatigue effect of crude polysaccharide of Floccularia luteovirens on mice was verified.To investigate the changes of metabolites in mice after fatigue exercise,the concentrations of lactate(LA),creatine kinase(CK),urea nitrogen(BUN)and malondialdehyde(MDA)in mice serum were measured by ELISA kits in this study.The concentration of reactive oxygen species(ROS)and the activities of three antioxidant enzymes,namely superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px),were also measured in mice in this study.The experimental results showed that the body weight and liver index of mice in the drug-administered group were increased compared with those in the blank control group and the model group.Compared with the mice in the model group,the mice in the drug administration group showed a significant increase in the fatigue rotation time and a significant decrease in the resting time of forced swimming in mice,and all of them showed a dose-dependent relationship.The serum metabolites lactate(LA),creatine kinase(CK),urea nitrogen(BUN),malondialdehyde(MDA)and reactive oxygen species(ROS)in mice in the model group were significantly increased after forced exercise compared with the blank control group,and all these values were gradually reduced to normal after the administration of crude polysaccharide of Floccularia luteovirens.The concentrations of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in the mice of the model group were significantly decreased after forced exercise,and the antioxidant enzyme activities gradually increased after administration,and the antioxidant enzyme activities were significantly increased after high dose administration compared with the blank control group.In conclusion,this study demonstrated that the crude polysaccharide of Floccularia luteovirens can improve the accumulation of metabolites BUN,LA,CK,MDA and ROS in mice after fatigue exercise;increase the activity of antioxidant enzymes SOD,CAT and GSH-Px.Thus,it can be confirmed that the crude polysaccharide of Floccularia luteovirens has anti-fatigue effect.