Development Of An M7G-related Gene Prognostic Model For Predicting Overall Survival In Patients With Lung Adenocarcinoma And Characterization Of The Tumor Microenvironment

Objective:Lung adenocarcinoma is a common type of lung cancer characterized by poor prognosis and high mortality.However,recent studies have shown that N7-methylguanosine(m7G)has an increasingly critical role in different types of tumors.Therefore,our study revolved around lung adenocarcinoma and the N7-methylguanosine-related gene(m7GRG).Methods:Using the gene expression and clinical data of TCGA and GEO databases,the genes associated with the prognosis of lung adenocarcinoma were screened out by COX regression analysis and least absolute shrinkage selection operator(LASSO)regression analysis,and a risk model for predicting prognosis was established.By Kaplan-Meier analysis,the prognosis of high-risk and low-risk groups was determined,and the difference in pharmacological sensitivity between the two groups was further evaluated.Cell experiments and qrtPCR validation were performed to create and validate a 29 m7GRG-based model for predicting the prognosis of LUAD patients.Results:These 29 m7GRG models were identified as important prognostic factors that could be used to guide clinical treatment.In addition,our functional studies found that m7GRG was mainly enriched in cancer-related signaling pathways,which suggested that m7GRG may be involved in the occurrence and development of LUAD.In addition,we also found that the m7GRG model is related to the infiltration of immune cells and immune checkpoints,suggesting that m7GRG may be related to immunotherapy and chemotherapy,providing guidance for these treatments.At the same time,the study found that knockout of the SPINK1 gene affects lung adenocarcinoma cells value addition,invasion and migration.Conclusion:Based on the above results,our study shows that m7GRG is closely related to the prognosis of LUAD,which can be used to predict the survival status of patients and provide guidance for immunotherapy and chemotherapy of lung adenocarcinoma.In the future,we will further study the molecular mechanism and clinical application value of m7GRG to better guide the diagnosis and treatment of lung adenocarcinoma.