The Association Of Cellular Prion Protein With CD4~+T Lymphocyte Subsets In The Blood In Cerebral Ischemic Stroke

Objective:In order to investigate the plasma level of PrPC and CD4+T lymphocyte subsets after cerebral ischemic stroke.Meanwhile,the association of the PrPC with the CD4+T lymphocyte subsets was analyzed.Methods:1、From May 2022 to December 2022,we recruited a total of 42 patients who were suffering from anterior circulation cerebral ischemic stroke within 72 hours of onset from the Department of Neurology,in the first hospital of Jilin university.All patients were selected by the inclusion and exclusion criteria.We divided the patients into mild stroke group(NIHSS≤6,n=24 cases)and moderate to severe stroke group(NIHSS>6,n=18 cases).At the same time,24 volunteers from the physical examination center were recruited as the control group.The baseline information of patients and controls,such as gender,age,NIHSS score,hypertension,diabetes et al was recorded.On the second morning of admission,venous blood was collected on an empty stomach,as well as in the control group.We used the Flow cytometry to test the CD4+T cell subsets.The plasma level of PrPC were detected by ELISA.2、We compared the plasma level of PrPC between the stroke group and the control group,the same as the mild stroke group and the moderate to severe stroke group.3、The CD4+T cell subsets were compared between the stroke group and the control group,the same as the mild stroke group and the moderate to severe stroke group.4、The association of the plasma level of PrPC with the subsets of CD4+T cells was analyzed.Results:1、Compared with the control group,The plasma level of PrPC in the stroke group was higher(P<0.001).Meanwhile,the PrPC content in the moderate to severe stroke group was higher than that in the mild stroke group(P<0.05).2、Compared with the control group,the Th2 and Th17 subsets in CD4+T lymphocytes were both higher while the ratio of Thl/Th2 cell was lower in the stroke group(P<0.01,P<0.05),(P<0.01).The Th1 subset in CD4+T lymphocytes was slightly higher,but the result didn’t make sense(P>0.05).Compared with the mild stroke group,the ratios of Th1,Th2 and Th17 in CD4+T lymphocytes were increased and the ratio of Th1/Th2 cell was decreased in patients with moderate to severe stroke,but the result was meaningless(P>0.05).3、The Th2 subset in the CD4+T lymphocytes was positively correlated with the plasma level of PrPC in the stroke group(r=0.4367,P=0.0038),while there was no significant correlation between the Thl or Th17 subsets in the CD4+T lymphocytes or the ratio of Thl/Th2 cells and the plasma level of PrPC.In the non-stroke group,the CD4+T cell subsets weren’t relevant to the PrPC.Conclusions:1、Patients suffering from cerebral ischemic stroke had increased plasma level of PrPC.The level of PrPC was higher when the nerve injury was more severe.2、Patients suffering from cerebral ischemic stroke had increased Th2 and Th17 subsets in CD4+T lymphocytes and increased ratio of Th1/Th2 cells.3、The Th2 subset in CD4+T lymphocytes was positively correlated with the plasma level of PrPC in cerebral ischemic stroke.