Expression And The Effect On Prognosis Of Osteopontin And HMGB1 In Patients With Sepsis

Objective:In recent years,it has been found that in many infectious diseases,the expression of osteopontin(OPN)and high mobility group protein B1(HMGB1)will be increased to varying degrees.In addition,in hepatitis C virus-induced liver fibrosis,the expression of the two is correlated,OPN can induce HMGB1 and regulate the synthesis of type I collagen in hepatic stellate cells.Moreover,existing cell tests have shown that OPN is involved in regulating the expression of HMGB1 in the cell model of sepsis.However,it remains unclear whether the expression levels of OPN and HMGB1 are increased in patients with sepsis and whether OPN is involved in regulating the expression of HMGB1 in patients with sepsis.Therefore,this study aims to verify whether the expression levels of OPN and HMGB1 are increased in patients with sepsis,analyze whether they are correlated,and evaluate whether the expression levels of OPN and HMGB1 have predictive value for the prognosis of patients with sepsis.Methods:A total of 100 sepsis patients admitted to the emergency Department of Xiangya Hospital from January 2021 to September 2021 were selected as the experimental group.There were 63 male patients and 37 female patients.Nineteen healthy adults were selected from the physical examination center of Xiangya Hospital.There were 9 males and 10 females.The concentrations of OPN and HMGB1 in the serum of the experimental group and the control group were detected by enzyme linked immunosorbent assay(ELISA).The clinical data of the patients were collected in detail,and the Apache-Ⅱ score and SOFA score were calculated.R 4.1.3 software was used for statistical analysis of experimental data.Continuous variables were analyzed by Mann-Whitney U test,categorical variables were analyzed by Chi-square test,and data consistent with normal distribution were compared between groups by T test.If the measurement data meet the normal distribution,it is represented by the mean ± standard deviation(??±S);if not,it is represented by the median [M(Q1,Q3)].The predictive factors of 7-day and 28-day adverse outcomes of sepsis were further determined by logistic regression analysis.Spearman correlation analysis was used to analyze the correlation between OPN and HMGB1.ROC curves of APACHE-ⅱ,OPN and HMGB1 were drawn,and the area under ROC curve(AUC)values and the best truncation values of the three were calculated.The areas under ROC curve were compared by Delong method.α=0.05 was taken as the test level,and P < 0.05 was considered statistically significant.Results:The expression level of OPN in sepsis patients was significantly higher than that in control group(7.76ng/m L vs 1.29ng/m L,P <0.001).The expression level of HMGB1 in sepsis patients was significantly higher than that in the control group(238.30ng/ml vs 15.12ng/ml,P < 0.001).The expression levels of OPN and HMGB1 were positively correlated in patients with sepsis(R =0.402,P < 0.001).OPN had a high predictive value for 30-day adverse outcomes in patients with sepsis,showing a good OC-ROC area(area under the ROC curve was0.74).Conclusion:The increased expression of OPN and HMGB1 in patients with sepsis may be used as biomarkers for early sepsis diagnosis,and the increase of OPN and HMGB1 may be positively correlated.OPN may be a good indicator for predicting 7-day mortality and 28-day mortality in patients with sepsis,and HMGB1 may be a good indicator for predicting28-day mortality in patients with sepsis.