The Expression And Significance Of SDC2 And P53 In Crc Tissue

Objective:To explore the SDC2 methylation and p53 deletion or mutation in colorectal cancer tissues and their relationship with each clinicopathological feature of colorectal cancer,the correlation between SDC2 expression and p53 expression.It provides possible biological targets for colorectal cancer patients,thus exploring a new therapeutic strategy.Methods:The tissue paraffin specimens and the corresponding paracancerous normal tissues(non-cancerous normal intestinal mucosa>5 cm from the tumor margin)of 49 patients who attended the Department of Gastrointestinal Surgery of Cangzhou People’s Hospital and underwent radical colorectal surgery from January 2021 to December 2021 and were diagnosed as colorectal cancer in the postoperative pathology were selected for this study.Both colorectal cancer tissues and normal paracancerous tissues were subjected to SDC2 for fluorescent PCR and p53immunohistochemical detection.In this study,by observing the SDC2methylation and p53 deletion or mutation in 49 colorectal cancer tissues and their relationship with the clinical characteristics of colorectal cancer patients,the specimens were grouped according to whether choroidal infiltration occurred or not,and divided into the group with and without choroidal infiltration,and the data of the two groups were compared to screen out the presence of choroidal infiltration in colorectal cancer patients by means of single-factor analysis and multifactor analysis The independent influencing factors of To investigate the correlation between SDC2 expression and p53expression.Results:1.In colorectal cancer tissues,43 cases(87.76%)were positive for SDC2 and 6 cases(12.24%)were negative,while in normal tissues adjacent to cancer,3 cases(0.06%)were positive for SDC2 and 46 cases(99.94%)were negative.the positivity rate of SDC2 in colorectal cancer was higher than that in normal tissues adjacent to cancer,and the difference was statistically significant(P<0.01);the expression of SDC2 was correlated with The expression of SDC2 correlated with histological grading and vascular infiltration(P<0.05),while the expression of SDC2 did not correlate with patient’s gender,age,tumor diameter size,TNM stage,lymph node metastasis and nerve infiltration(P>0.05).2.In colorectal cancer tissues,29 cases(59.18%)were positive for p53and 20 cases(40.82%)were negative,while in normal tissues adjacent to cancer,0 cases(0%)were positive for p53 and 49 cases(100%)were negative.p53 positivity rate in colorectal cancer was higher than that in normal tissues adjacent to cancer,and the difference was statistically significant(P<0.01).p53 expression was associated with tumor diameter size,vascular infiltration were correlated(P<0.05),while the expression of p53 was not correlated with patient’s gender,age,TNM stage,histological grade,lymph node metastasis,and nerve infiltration(P<0.05).3.The analysis of all relevant factors,with the presence of vascular infiltration as the dependent variable,showed that TNM stage,lymph node metastasis,SDC2 expression,and p53 expression,may be associated with the presence of vascular infiltration in colorectal cancer(P<0.05);the multifactorial analysis of TNM stage,lymph node metastasis,SDC2expression,and p53 expression showed that p53 expression became The results showed that p53 expression was an independent risk factor for the development of vascular infiltration in colorectal cancer(P<0.05).4.The correlation between SDC2 and p53 expression was analyzed by Spearman correlation,and the expression of SDC2 was positively correlated with the expression of p53(r_s=0.323,P<0.05).Conclusions:The positive rate of SDC2 expression in colorectal cancer tissues was87.76%,which was closely related to the histological grade and vascular infiltration of colorectal cancer tissues;the positive rate of p53 expression in colorectal cancer tissues was 59.18%,which was closely related to the size of tumor diameter and vascular infiltration of colorectal cancer tissues;p53expression was an independent risk factor for the appearance of vascular infiltration in colorectal cancer;the expression of SDC2 was positively correlated with The expression of SDC2 was positively correlated with the expression of p53.