| Objective: In this study,the expression of miRNA-423-5p in plasma exosomes from Gastric stromal tumor patients and healthy humans was detected by q RT-PCR,and the relationship between it and clinical data was analyzed.Methods: A total of 48 patients with gastric stromal tumor diagnosed in the Second Affiliated Hospital of Anhui Medical University from October 2020 to February 2020,including 18 female,30 male and 10 healthy patients in the control group.Plasma exosomes were extracted from gastric stromal tumor patients and healthy people by the overspeed filtration method.The characteristics of the extracted plasma exosomes were identified by transmission electron microscopy,Nanoparticle Tracking Analysis,and Western Blot.The expression levels of miRNA-423-5p in plasma exosomes were determined by real-time quantitative PCR.Relevant clinical data were collected from patients with gastric stromal tumor,including patient sex,age,tumor size,nuclear division image,and risk grade.The relationship between expression level and clinical data was analyzed using SPSS26.0 statistical software,measured using data(x ±s),and the group was compared by t-test.P(27)0.05 was considered to be statistically significant differences.Results: The expression levels of miRNA-423-5p in plasma exosomes were significantly increased in gastric stromal tumor patients compared with normal controls(P<0.05),and its expression level was not correlated with patient sex and age(P> 0.05),and positively correlated with tumor diameter,mitotic image and risk(P <0.05).Conclusion: The expression of miRNA-423-5p was significantly up-regulated in exosomes of gastric GISTs.There was a positive correlation with tumor diameter,nuclear division image and risk.Moreover,high expression of miRNA-423-5p and poor prognosis factors are associated with stromal tumors,suggesting that exosomal miRNA-423-5p may be associated with poor prognosis and may become a marker for the diagnosis and prognosis of gastric stromal tumor. |
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