| Objective:To investigate the status quo of sleep quality and related factors of young people in cold cities in Harbin by sending questionnaires,and to understand the occurrence of sleep disorders under different constitutions of traditional Chinese medicine.By observing the behavioral changes and serum 5-HT and NE levels of insomnia rats under acupuncture cold factor,the influence of cold factor on sleep of rats was explored,which could provide reference for clinical treatment of sleep disorders in cold region.Method:1.From June 2021 to June 2022,a questionnaire survey was conducted among permanent young residents aged 18-45 years old in Harbin City.General information of the respondents was collected,and their sleep quality was evaluated with Pittsburgh Sleep Index scale and their physical conditions were analyzed with traditional Chinese Medicine Physique Scale.Through statistical analysis,the sleep quality and related influencing factors of young people in cold cities were analyzed,and the distribution of traditional Chinese medicine physique of young people in cold cities was obtained.2.Fifty Wistar male rats were randomly divided into 5 groups: blank group,modeling group,cold group,cold modeling group and acupuncture treatment group,with 10 rats in each group.The 5 groups of rats were fed for15 days under two different environments,in which the blank group and the modeling group were fed conventionally,and the cold group,the cold modeling group and the acupuncture treatment group were fed in a-10±1℃constant temperature refrigerator at the same time every day for 1.5h.15 days later,PCPA modeling was carried out in the modeling group,cold modeling group and acupuncture treatment group.At the end of modeling,the general condition and open field experimental results of the five groups of rats were recorded,and the changes of anxiety state of the rats in each group were observed.After completion of acupuncture treatment,the acupuncture treatment group was performed.0.35×20mm millimeter needle was selected to remove the pulse and perform twisting and purging method according to the Hai acupoint,with twisting amplitude < 90/ times and twisting frequency < 60 times /min.The twisting amplitude of the purging method was 90°-180°/ time,the twisting frequency was 60-90 times /min,the twisting duration was lmin(the rotation frequency of the watch second hand was used to control the rotation),and the needle was left for 30min(this operation was completed by the same right-handed experimental member),once a day,for a total of 7d.The other four groups were not treated and were given binding stimulation with the same duration and intensity as the acupuncture treatment group.After the treatment,blood was collected from the abdominal aorta of rats,and serum5-HT and NE concentrations in each group were measured.The differences among the groups were compared to observe the effect of acupuncture on insomnia rats under cold factors.HE staining was performed to observe the pathological morphological changes of rat neurons.Results:1.A total of 632 effective investigations were completed in this survey.According to PSQI score results,the incidence of poor sleep quality was31.01%;There were statistical differences in the incidence of poor sleep quality among different age,BMI,smoking history,family history of insomnia and season(P < 0.05),while there were no statistical differences in the incidence of poor sleep quality among different genders and education levels(P > 0.05).2.The survey results of TCM constitution scale showed that Yin deficiency was the highest occurrence frequency of TCM constitution in the poor sleep quality group(PSQI≥8);In the group with good sleep quality(PSQI < 8),the highest frequency of TCM constitution was peaceful quality.The incidence of poor sleep quality was different in different constitutions.The incidence of poor sleep quality in Yin deficiency,Qi stagnation and Yang deficiency was significantly higher than that in other constitutions,while the incidence of poor sleep quality in peaceful type was significantly lower than that in other constitutions(P < 0.001).3.After modeling,compared with the blank group,the hair of the cold group,the modeling group,the cold group and the acupuncture treatment group was dull and messy,the urine and feces increased,the body weight decreased,and the mental state decreased.The open field experiment showed that the rest time of the modeling group and the cold group was significantly higher than that of the blank group(P < 0.001),the rest time of the cold modeling group and the acupuncture treatment group was higher than that of the modeling group and the cold group(P < 0.05),and the rest time of the cold modeling group and the acupuncture treatment group had no significant difference(P > 0.05).The movement distance of the central region in the modeling group and the cold group was significantly lower than that in the blank group(P < 0.001),the movement distance of the central region in the cold modeling group and the acupuncture treatment group was lower than that in the modeling group and the cold group(P < 0.05),but there was no statistical difference between the cold modeling group and the acupuncture treatment group(P > 0.05).4.After treatment,the hair of rats in the acupuncture treatment group was smooth,the color of feces turned black,the mental state was good,the squirrel cage was dry,and the body weight increased.Serum 5-HT and NE showed that the content of 5-HT and NE in acupuncture treatment group was higher(P < 0.001)and lower(P < 0.001)than that in cold modeling group.Compared with blank group,5-HT content was decreased(P < 0.001)and NE content was increased(P < 0.001)in cold group.Conclusion:1.The incidence of poor sleep quality among young people in cold areas was 31.01%,and age,BMI,smoking history,family history of insomnia and season were the influencing factors.Young people in cold areas with Yin deficiency,Qi stagnation and Yang deficiency were more likely to have poor sleep quality.2.The cold factor can affect the sleep status of rats and aggravate the anxiety of rats.3.Acupuncture can increase the content of serum 5-HT and decrease the content of NE in rats with insomnia under cold factors,which can improve their sleep. |
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