Proteomics-based DIA Technology Screens Differential Proteins For Abdominal Aortic Calcification In Patients With Primary Aldosteronism

Objective: To screen serum differential proteins(EDPs)for abdominal aortic calcification(AAC)in patients with primary aldosteronism(PA)using a non-tagged proteomics approach technique.Methods: We used data-independent collection mass spectrometry to detect the serum of the two groups of people in the PA with AAC group and the PA without AAC group,and further analyzed the differentially expressed protein data with bioinformatics software.Results: A total of 38 differentially expressed proteins(6 up-regulated and 32 down-regulated)were screened in the serum specimens of both groups.Gene Ontology analysis indicated that these significant proteins mainly involved in extracellular space,extracellular regions and extracellular exosome;Biological processes mainly involve cell adhesion and immune response;Its molecular function is related to calcium binding and collagen binding.KEGG analysis showed that the differentially expressed proteins were mainly involved in the EMC receptor interaction,Protein digestion and abserption and Focal adhestion pathways.Conclusions:There were significant differences in serum proteomics between patients with PA with AAC and those in the PA without AAC group.Among them,there was a reduced expression of many ECM proteins important for collagen structure and function,especially those involved in collagen fibril formation,and these proteins may be involved in the regulation of abdominal aortic calcification in PA.