Optimization And Control Of ANM 33-loaded Lipid Microbubbles

AIM:By observing the changes of drug concentration and time in the blood process of SD rats,the optimal drug administration concentration of anti-mi R 33 dual-target lipid microbubbles and the amount of drug loss in the process of reaching the target area were found,and the corresponding experimental basis and theoretical basis for future interference and treatment of AS.Methods:Preparation of dual-target lipid microbubbles carrying anti-mi R 33 and detection of their basic physical properties,Healthy male SD rats were randomly divided into two groups:(1)injected 7μg×10~8concentration loaded anti-mi R 33 lipid microbubble,(2)injected 3.5μg×10~8concentration(3)pbs control group,10 animals in each group,SD rats in the tail vein,Blood was collected through the orbital venous plexus at 10 time points within 24h,The plasma concentration of rats at each time point was determined by UV spectrophotometer(wavelength 550nm),Two sets of pharmacokinetic parameters were fitted using the DAS2.1.1 pharmacokinetic software,The results of the main pharmacokinetic parameters of the SD rats were then analyzed by SPSS17.0 statistical software.conclusion:The high concentration group has high peak concentration,fast peak reaching time,long duration in vivo,slow drug elimination rate,relatively low concentration group,high bioavailability,and better effect,which provides theoretical basis for later treatment.