MiR-142-5p Inhibits Osteogenic Differentiation Of Rat BMSCs By Targeting LHX8

Objective: Osteoporosis is a common systemic bone metabolic disease with high incidence and great harm.Enhancing the ability of osteogenic differentiation in clinical treatment is the main way to improve osteoporosis.However,the molecular mechanism of osteogenic differentiation of BMSCs in osteoporosis has not been fully understood.Therefore,the purpose of this study was to investigate the regulation of miR-142-5p and LIM Homeobox 8(LHX8)on osteogenic differentiation of BMSCs in the occurrence and development of osteoporosis.Methods: We first constructed animal models of osteoporosis(OVX)and sham operation group(NC).The total RNA of BMSCs in OVX group and NC group was extracted for whole transcriptome sequencing,and genes related to osteoporosis were selected.The expression levels of m RNA LHX8 and miR-142-5p in OVX group and NC group were verified by RT-PCR,and the expression level of LHX8 protein was verified by Western-Blot.The expression of LHX8 and miR-142-5p was detected during osteogenic differentiation to verify its role in osteogenic differentiation.The regulatory relationship between LHX8 and miR-142-5p was analyzed by bioinformatics and verified by double luciferase assay.BMSCs were transfected with miR-142-5p mimics and miR-NC(negative control)to further verify the role of miR-142-5p in osteogenic differentiation.Results: Differential analysis of transcriptome sequencing results showed that the expression levels of m RNA LHX8 and miR-142-5p were significantly different between OVX group and NC group.In addition,RT-PCR found that LHX8 expression was significantly decreased in the OVX group,while miR-142-5p was significantly up-regulated,and bioinformatics analysis found that miR-142-5p could target LHX8.The results of RT-PCR showed that the expression level of miR-142-5p increased steadily after transfection of BMSCs with miR-142-5p mimics,and it could significantly inhibit the osteogenic differentiation of BMSCs and the expression of LHX8.Dual fluorescein experiment verified that miR-142-5p could directly target LHX8.Therefore,miR-142-5p is involved in osteogenic differentiation of BMSCs by regulating LHX8 expression,which may be a potential diagnostic target for osteoporosis.Conclusions: MiR-142-5p can bind to LHX8 and participate in the regulation mechanism of inhibiting osteogenic differentiation of BMSCs.The osteogenic differentiation ability of BMSCs is weakened,which is involved in the process of promoting osteoporosis.It reflects the complexity and network regulation characteristics of osteoporosis regulation process.