Preparation Of SF/PVP Nanofiber Membrane Loaded With Puerarin And Its Skin Repair Effect

The skin is the largest organ of the body and plays a huge role in maintaining the body’s temperature and fluid balance,preventing microbial invasion,and intercepting physical and chemical damage.However,the skin is extremely vulnerable to damage.Different complications caused by skin defects such as bleeding,infection,and excessive inflammation can place a greater burden on wound healing,which in turn slows down the wound closure process.Skin wounds due to trauma or chronic diseases have a non-negligible impact on millions of patients worldwide.Therefore,a lot of research is needed to prepare wound dressings that can improve wound healing rates.Puerarin(PUE)is rich in pharmacological activity and has shown beneficial effects on a variety of dermatological conditions.Recently,researchers are working to develop drug-loaded nanofiber wound dressings with biocompatible and biodegradable properties,which have a large specific surface area and high porosity,with the original properties of the nanofiber structure and the excellent biological activity of the loaded drug.Therefore,this study aimed to develop a gerberosin-loaded composite nanofiber film(SF/PVP/PUE)using electrostatic spinning technology and to evaluate its ability to promote skin repair by in vivo and in vitro experiments,as follows.(1)Preparation characterization and performance study of puerarin composite nanofiber membranesThe composite nanofiber membranes loaded with puerarin were prepared by electrostatic spinning technique,and the physicochemical properties of the fibers were characterized to evaluate the changes in the properties of the fibers before and after drug loading and the effects of drug loading on the bioactivity of the fiber membranes.The results showed that the SF/PVP/PUE nanofiber membranes had uniform diameter distribution,high porosity,and good mechanical properties.After drug loading,the wettability and permeability of the fibers increased,and the antibacterial and antioxidant properties were significantly improved.The fiber membranes were biocompatible and could be safely used for subsequent experiments.(2)Study on the role of SF/PVP/PUE in protecting UVB-induced photodamage of Ha Ca T cellsUltraviolet radiation(UVB)can cause skin damage.In this study,we used UVB irradiation of Ha Ca T cells to induce skin damage in an in vitro model.We found that UVB irradiation for 5min could cause significant oxidative damage to Ha Ca T cells,with increased intracellular ROS levels,increased oxidative stress,and eventually apoptosis.In contrast,SF/PVP/PUE treatment significantly increased the viability of Ha Ca T cells after UVB irradiation,increased the activity of SOD,GSH-PX,and CAT antioxidant enzymes,and decreased ROS production.In addition,SF/PVP/PUE significantly downregulated NF-κB expression and secretion of IL-1β,TNF-α,COX-2,and i NOS,and attenuated the inflammatory response.Most importantly,we found that the protective effect of SF/PVP/PUE on UVB-injured Ha Ca T cells may promote Nrf2 nuclear translocation and activate the antioxidant system.(3)Study of the role of SF/PVP/PUE in attenuating LPS-induced macrophage inflammationFree radicals in non-healing wounds activate macrophages in the skin to produce an inflammatory response and accelerate tissue damage.In this study,we evaluated the antiinflammatory effects of SF/PVP/PUE in vitro using an LPS-induced macrophage inflammation model.We found that SF/PVP/PUE nanofibers had strong anti-inflammatory activity,reduced the secretion of IL-1β,TNF-α,COX-2,i NOS,and NO in LPS-induced macrophages,increased macrophage viability,and attenuated LPS-induced cellular inflammatory damage and apoptosis,and hypothesized that SF/PVP/PUE could be applied to the wound repair process in the presence of inflammation.(4)Study on the role of SF/PVP/PUE in promoting skin wound repair in miceThe skin repair effect of SF/PVP/PUE nanofibers was evaluated by constructing a mouse skin whole wound model.The animal experimental data showed that SF/PVP/PUE nanofiber treatment could promote wound contraction,increase the expression of CD31,PCNA and α-SMA at the wound site and decrease the production of CD68.Meanwhile,SF/PVP/PUE also accelerated wound healing by reducing the inflammatory response during wound repair.In conclusion,SF/PVP/PUE nanofibers are a very promising material for skin repair.